• Immunofluorescence analysis of A431 cells using CDH3 Rabbit polyclonal antibody (STJ116448) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of CDH3 in paraffin-embedded human skin using CDH3 Rabbit polyclonal antibody (STJ116448) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using CDH3 Rabbit polyclonal antibody (STJ116448) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CDH3 antibody (24-350) (STJ116448)

SKU:
STJ116448

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CDH3 (24-350) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDH3
Gene ID: 1001
Uniprot ID: CADH3_HUMAN
Immunogen Region: 24-350
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 24-350 of human CDH3 (NP_001784.2).
Immunogen Sequence: SEPCRAVFREAEVTLEAGGA EQEPGQALGKVFMGCPGQEP ALFSTDNDDFTVRNGETVQE RRSLKERNPLKIFPSKRILR RHKRDWVVAPISVPENGKGP FPQRLNQLKSNKDRDTKIFY SITGPGADSPPEGVFAVEKE TGWLLLNKPLDREEIAKYEL FGHAVSENGASVEDPMNISI IVTDQNDHKPKFTQDTFRGS VLEGVLPGTSVMQVTATDED DAIYTYNGVVAYSIHSQEP
Tissue Specificity Expressed in some normal epithelial tissues and in some carcinoma cell lines.
Function Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells.cadherins may thus contribute to the sorting of heterogeneous cell types.
Protein Name Cadherin-3
Placental Cadherin
P-Cadherin
Database Links Reactome: R-HSA-418990
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Cadherin-3 antibody
Anti-Placental Cadherin antibody
Anti-P-Cadherin antibody
Anti-CDH3 antibody
Anti-CDHP antibody

Information sourced from Uniprot.org

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