• Western blot analysis of extracts of various cell lines, using LI Cadherin/Cadherin-17 rabbit monoclonal antibody (STJ11102306) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded human gastric cancer using LI Cadherin/Cadherin-17 rabbit monoclonal antibody (STJ11102306) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Human pancreatic ductal carcinoma using LI Cadherin/Cadherin-17 rabbit monoclonal antibody (STJ11102306) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human stomach using LI Cadherin/Cadherin-17 rabbit monoclonal antibody (STJ11102306) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Confocal imaging of Human colon cancer using LI Cadherin/Cadherin-17 rabbit monoclonal antibody (STJ11102306, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x.
  • Immunofluorescence analysis of mouse Intestine using LI Cadherin/Cadherin-17 rabbit monoclonal antibody (STJ11102306) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat rectum using LI Cadherin/Cadherin-17 rabbit monoclonal antibody (STJ11102306) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-CDH17 antibody (1-100) [S6MR] (STJ11102306)

SKU:
STJ11102306

$197.78 - $867.38
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:2000
IHC-P:1:200-1:2000
IF/ICC:1:100-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDH17
Gene ID: 1015
Uniprot ID: CAD17_HUMAN
Immunogen Region: 1-100
Specificity: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human LI Cadherin/Cadherin-17 (Q12864).
Immunogen Sequence: MILQAHLHSLCLLMLYLATG YGQEGKFSGPLKPMTFSIYE GQEPSQIIFQFKANPPAVTF ELTGETDNIFVIEREGLLYY NRALDRETRSTHNLQVAALD
Tissue Specificity Expressed in the gastrointestinal tract and pancreatic duct. Not detected in kidney, lung, liver, brain, adrenal gland and skin.
Function Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells.cadherins may thus contribute to the sorting of heterogeneous cell types. LI-cadherin may have a role in the morphological organization of liver and intestine. Involved in intestinal peptide transport.
Protein Name Cadherin-17
Intestinal Peptide-Associated Transporter Hpt-1
Liver-Intestine Cadherin
Li-Cadherin
Database Links Reactome: R-HSA-418990
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Cadherin-17 antibody
Anti-Intestinal Peptide-Associated Transporter Hpt-1 antibody
Anti-Liver-Intestine Cadherin antibody
Anti-Li-Cadherin antibody
Anti-CDH17 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance