HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CDH11 with C-terminal MYC tag probed with STJ72255 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with STJ72255 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.

Anti-CDH11 antibody (26-37) (STJ72255)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
STJ72255-100
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General Information

Short DescriptionGoat polyclonal antibody anti-CDH11 (26-37) is suitable for use in ELISA and Western Blot research applications.
ApplicationsPep-ELISA/WB-Trf
HostGoat
ReactivityHuman/Mouse/Rat/Dog/Cow
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration0.5 mg/mL
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution RangePeptide ELISA: antibody detection limit dilution 1:2000.
WB: In transfected HEK293 transiently expressing full-length Human CDH11 (myc and DYKDDDDK tagged) , a band of approx. 130kDa was observed. No bands were observed in mock-transfected HEK293
Formulation0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage InstructionStore at-20ยฐC on receipt and minimise freeze-thaw cycles.

Target Information

Gene SymbolCDH11
Gene ID1009
Uniprot IDCAD11_HUMAN
Accession NumberNP_001788.2
Immunogen Region26-37
Immunogen SequenceERRGHLRPSFHG

Additional Info

Function Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells.cadherins may thus contribute to the sorting of heterogeneous cell types. Required for proper focal adhesion assembly. Involved in the regulation of cell migration.
Protein Name Cadherin-11
Osf-4
Osteoblast Cadherin
Ob-Cadherin
Database Links Reactome: R-HSA-418990
Reactome: R-HSA-9759811
Reactome: R-HSA-9762292
Reactome: R-HSA-9833576
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Cadherin-11 antibody
Anti-Osf-4 antibody
Anti-Osteoblast Cadherin antibody
Anti-Ob-Cadherin antibody
Anti-CDH11 antibody

Information sourced from Uniprot.org

Citations

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