Anti-CDCA2 antibody (511-560 aa) (STJ95418)

SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human CDCA2 at the amino acid range 511-560
STJ95418
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General Information

Short DescriptionRabbit polyclonal anti-Cell division cycle-associated protein 2 (511-560 aa) for use in WB, IHC, IF and ELISA in Human samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC/IF/ELISA
HostRabbit
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration1 mg/mL
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Dilution RangeWB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
FormulationLiquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolCDCA2
Gene ID157313
Uniprot IDCDCA2_HUMAN
ImmunogenThe antiserum was produced against synthesized peptide derived from the human CDCA2 at the amino acid range 511-560
Immunogen Region511-560 aa
SpecificityRepo-Man Polyclonal Antibody detects endogenous levels of Repo-Man protein.

Additional Info

Post Translational Modifications Phosphorylated by CDK1. May regulate its subcellular location.
Function Regulator of chromosome structure during mitosis required for condensin-depleted chromosomes to retain their compact architecture through anaphase. Acts by mediating the recruitment of phopsphatase PP1-gamma subunit (PPP1CC) to chromatin at anaphase and into the following interphase. At anaphase onset, its association with chromatin targets a pool of PPP1CC to dephosphorylate substrates.
Protein Name Cell Division Cycle-Associated Protein 2
Recruits Pp1 Onto Mitotic Chromatin At Anaphase Protein
Repo-Man
Database Links
Cellular Localisation Nucleus
Excluded From The Nucleolus
Present In Nucleoplasm Throughout The G1
S And G2 Stages Of The Cell Cycle
During M Phase
It Becomes Diffuse Throughout The Cell As The Nuclear Membrane Breaks Down
And Faintly Accumulates Later On Metaphase Chromatin
As The Cell Progresses To Anaphase
It Accumulates On Chromatin
Alternative Antibody Names Anti-Cell Division Cycle-Associated Protein 2 antibody
Anti-Recruits Pp1 Onto Mitotic Chromatin At Anaphase Protein antibody
Anti-Repo-Man antibody
Anti-CDCA2 antibody

Information sourced from Uniprot.org

Citations

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