• Western blot analysis of lysates from 293 cells, using CDCA2 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using Repo-Man Polyclonal Antibody diluted at 1：2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-CDCA2 antibody (511-560 aa) (STJ95418)

SKU:
STJ95418

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Cell division cycle-associated protein 2 (511-560 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDCA2
Gene ID: 157313
Uniprot ID: CDCA2_HUMAN
Immunogen Region: 511-560 aa
Specificity: Repo-Man Polyclonal Antibody detects endogenous levels of Repo-Man protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human CDCA2 at the amino acid range 511-560
Post Translational Modifications Phosphorylated by CDK1. May regulate its subcellular location.
Function Regulator of chromosome structure during mitosis required for condensin-depleted chromosomes to retain their compact architecture through anaphase. Acts by mediating the recruitment of phopsphatase PP1-gamma subunit (PPP1CC) to chromatin at anaphase and into the following interphase. At anaphase onset, its association with chromatin targets a pool of PPP1CC to dephosphorylate substrates.
Protein Name Cell Division Cycle-Associated Protein 2
Recruits Pp1 Onto Mitotic Chromatin At Anaphase Protein
Repo-Man
Cellular Localisation Nucleus
Excluded From The Nucleolus
Present In Nucleoplasm Throughout The G1
S And G2 Stages Of The Cell Cycle
During M Phase
It Becomes Diffuse Throughout The Cell As The Nuclear Membrane Breaks Down
And Faintly Accumulates Later On Metaphase Chromatin
As The Cell Progresses To Anaphase
It Accumulates On Chromatin
Alternative Antibody Names Anti-Cell Division Cycle-Associated Protein 2 antibody
Anti-Recruits Pp1 Onto Mitotic Chromatin At Anaphase Protein antibody
Anti-Repo-Man antibody
Anti-CDCA2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance