Anti-CDC25B antibody (100-200) [S2MR] (STJ11102542)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal Cdc25B (100-200) antibody for use in WB, IHC-P and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S2MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CDC25B |
| Gene ID | 994 |
| Uniprot ID | MPIP2_HUMAN |
| Immunogen Region | 100-200 |
| Immunogen Sequence | SSESSESSDAGLCMDSPSPM DPHMAEQTFEQAIQAASRII RNEQFAIRRFQSMPVRLLGH SPVLRNITNSQAPDGRRKSE AGSGAASSSGEDKENDGFVF K |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human CDC25B (P30305). |
Additional Info
| Post Translational Modifications | Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-353 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-169 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-323 and Ser-375 by MAPK14 is required for binding to 14-3-3 proteins. |
| Function | Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. The three isoforms seem to have a different level of activity. |
| Protein Name | M-Phase Inducer Phosphatase 2Dual Specificity Phosphatase Cdc25b |
| Database Links | Reactome: R-HSA-69273Reactome: R-HSA-69656Reactome: R-HSA-8862803Reactome: R-HSA-9825892 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeSpindle Pole |
| Alternative Antibody Names | Anti-M-Phase Inducer Phosphatase 2 antibodyAnti-Dual Specificity Phosphatase Cdc25b antibodyAnti-CDC25B antibodyAnti-CDC25HU2 antibody |
Information sourced from Uniprot.org