• Flow cytometry:1X10^6 C2C12 cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (2 Mu g/mL, left) or CD80 Rabbit monoclonal antibody (STJ11105206, 2 Mu g/mL, right).
  • Flow cytometry:1X10^6 Neuro-2a cells (negative control, left) and C2C12 cells (right) were surface-stained with CD80 Rabbit monoclonal antibody (STJ11105206, 2 Mu g/mL, orange line) or ABflo® 647 Rabbit IgG isotype control (2 Mu g/mL, blue line). Non-fluorescently stained cells was used as blank control (red line).
  • Western blot analysis of various lysates, using CD80 Rabbit monoclonal antibody (STJ11105206) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of various lysates, using CD80 Rabbit monoclonal antibody (STJ11105206) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.

Anti-CD80 antibody (38-245) [S5206RM] (STJ11105206)

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STJ11105206

$197.78 - $863.33
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Host: Rabbit
Applications: WB/FC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CD80 (38-245) is suitable for use in Western Blot and Flow Cytometry research applications.
Clonality: Monoclonal
Clone ID: S5206RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
FC 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Cd80
Gene ID: 12519
Uniprot ID: CD80_MOUSE
Immunogen Region: 38-245
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 38-245 of mouse CD80 (NP_033985.3)
Immunogen Sequence: VDEQLSKSVKDKVLLPCRYN SPHEDESEDRIYWQKHDKVV LSVIAGKLKVWPEYKNRTLY DNTTYSLIILGLVLSDRGTY SCVVQKKERGTYEVKHLALV KLSIKADFSTPNITESGNPS ADTKRITCFASGGFPKPRFS WLENGRELPGINTTISQDPE SELYTISSQLDFNTTRNHTI KCLIKYGDAHVSEDFTWEKP PEDPPDSK
Tissue Specificity Expressed on activated B-cells, gamma interferon stimulated monocytes and non-circulating B-cell malignancies.
Function Involved in the costimulatory signal essential for T lymphocytes activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.
Protein Name T-Lymphocyte Activation Antigen Cd80
Activation B7-1 Antigen
B7
Cd Antigen Cd80
Database Links Reactome: R-MMU-1257604
Reactome: -MMU-389356
Reactome: -MMU-389357
Reactome: -MMU-389359
Reactome: -MMU-389513
Reactome: -MMU-6811558
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-T-Lymphocyte Activation Antigen Cd80 antibody
Anti-Activation B7-1 Antigen antibody
Anti-B7 antibody
Anti-Cd Antigen Cd80 antibody
Anti-Cd80 antibody
Anti-B7 antibody

Information sourced from Uniprot.org

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