Anti-CD27 antibody (20-191) (STJ118100)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal CD27 (20-191) antibody for use in WB and ELISA in mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CD27 |
| Gene ID | 939 |
| Uniprot ID | CD27_HUMAN |
| Immunogen Region | 20-191 |
| Immunogen Sequence | ATPAPKSCPERHYWAQGKLC CQMCEPGTFLVKDCDQHRKA AQCDPCIPGVSFSPDHHTRP HCESCRHCNSGLLVRNCTIT ANAECACRNGWQCRDKECTE CDPLPNPSLTARSSQALSPH PQPTHLPYVSEMLEARTAGH MQTLADFRQLPARTLSTHWP PQRSLCSSDFIR |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 20-191 of human CD27 (NP_001233.1). |
Additional Info
| Tissue Specificity | Found in most T-lymphocytes. |
| Post Translational Modifications | Phosphorylated. N-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. |
| Function | Costimulatory immune-checkpoint receptor expressed at the surface of T-cells, NK-cells and B-cells which binds to and is activated by its ligand CD70/CD27L expressed by B-cells. The CD70-CD27 signaling pathway mediates antigen-specific T-cell activation and expansion which in turn provides immune surveillance of B-cells. Mechanistically, CD70 ligation activates the TRAF2-PTPN6 axis that subsequently inhibits LCK phosphorylation to promote phenotypic and transcriptional adaptations of T-cell memory. In addition, activation by CD70 on early progenitor cells provides a negative feedback signal to leukocyte differentiation during immune activation and thus modulates hematopoiesis. Negatively regulates the function of Th2 lymphocytes in the adipose tissue. |
| Protein Name | Cd27 AntigenCd27l ReceptorT-Cell Activation Antigen Cd27T14Tumor Necrosis Factor Receptor Superfamily Member 7Cd Antigen Cd27 |
| Database Links | Reactome: R-HSA-5669034 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-Cd27 Antigen antibodyAnti-Cd27l Receptor antibodyAnti-T-Cell Activation Antigen Cd27 antibodyAnti-T14 antibodyAnti-Tumor Necrosis Factor Receptor Superfamily Member 7 antibodyAnti-Cd Antigen Cd27 antibodyAnti-CD27 antibodyAnti-TNFRSF7 antibody |
Information sourced from Uniprot.org