• Immunofluorescence analysis of HeLa cells using CD1D Rabbit polyclonal antibody (STJ22977). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates, using CD1D Rabbit polyclonal antibody (STJ22977) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CD1D antibody (20-190) (STJ22977)

SKU:
STJ22977

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CD1D (20-190) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD1D
Gene ID: 912
Uniprot ID: CD1D_HUMAN
Immunogen Region: 20-190
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-190 of human CD1D (NP_001757.1).
Immunogen Sequence: EVPQRLFPLRCLQISSFANS SWTRTDGLAWLGELQTHSWS NDSDTVRSLKPWSQGTFSDQ QWETLQHIFRVYRSSFTRDV KEFAKMLRLSYPLELQVSAG CEVHPGNASNNFFHVAFQGK DILSFQGTSWEPTQEAPLWV NLAIQVLNQDKWTRETVQWL LNGTCPQFVSG
Tissue Specificity Expressed on cortical thymocytes, on certain T-cell leukemias, and in various other tissues.
Function Antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on natural killer T-cells.
Protein Name Antigen-Presenting Glycoprotein Cd1d
R3g1
Cd Antigen Cd1d
Database Links Reactome: R-HSA-198933
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Basolateral Cell Membrane
Endosome Membrane
Lysosome Membrane
Endoplasmic Reticulum Membrane
Subject To Intracellular Trafficking Between The Cell Membrane
Endosomes And Lysosomes
Alternative Antibody Names Anti-Antigen-Presenting Glycoprotein Cd1d antibody
Anti-R3g1 antibody
Anti-Cd Antigen Cd1d antibody
Anti-CD1D antibody

Information sourced from Uniprot.org

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