• Immunohistochemistry analysis of CD151 in paraffin-embedded human lung cancer using CD151 Rabbit polyclonal antibody (STJ22974) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of CD151 in paraffin-embedded rat kidney using CD151 Rabbit polyclonal antibody (STJ22974) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Rat uterus, using CD151 Rabbit polyclonal antibody (STJ22974) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CD151 antibody (113-253) (STJ22974)

SKU:
STJ22974

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CD151 (113-253) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD151
Gene ID: 977
Uniprot ID: CD151_HUMAN
Immunogen Region: 113-253
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 113-253 of human CD151 (NP_004348.2).
Immunogen Sequence: AYYQQLNTELKENLKDTMTK RYHQPGHEAVTSAVDQLQQE FHCCGSNNSQDWRDSEWIRS QEAGGRVVPDSCCKTVVALC GQRDHASNIYKVEGGCITKL ETFIQEHLR
Tissue Specificity Expressed in a variety of tissues including vascular endothelium and epidermis. Expressed on erythroid cells, with a higher level of expression in erythroid precursors than on mature erythrocytes.
Post Translational Modifications Palmitoylated. Palmitoylation by ZDHHC2 regulates CD151 expression, association with other tetraspanin family proteins and function in cell adhesion.
Function Essential for the proper assembly of the glomerular and tubular basement membranes in kidney. (Microbial infection) Plays a role in human papillomavirus 16/HPV-16 endocytosis upon binding to cell surface receptor.
Protein Name Cd151 Antigen
Gp27
Membrane Glycoprotein Sfa-1
Platelet-Endothelial Tetraspan Antigen 3
Peta-3
Tetraspanin-24
Tspan-24
Cd Antigen Cd151
Database Links Reactome: R-HSA-2022090
Reactome: R-HSA-446107
Cellular Localisation Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Cd151 Antigen antibody
Anti-Gp27 antibody
Anti-Membrane Glycoprotein Sfa-1 antibody
Anti-Platelet-Endothelial Tetraspan Antigen 3 antibody
Anti-Peta-3 antibody
Anti-Tetraspanin-24 antibody
Anti-Tspan-24 antibody
Anti-Cd Antigen Cd151 antibody
Anti-CD151 antibody
Anti-TSPAN24 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance