Anti-CCNA2 antibody (350-432) (STJ29947)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Cyclin A2 (350-432) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CCNA2 |
| Gene ID | 890 |
| Uniprot ID | CCNA2_HUMAN |
| Immunogen Region | 350-432 |
| Immunogen Sequence | YLPSVIAGAAFHLALYTVTG QSWPESLIRKTGYTLESLKP CLMDLHQTYLKAPQHAQQSI REKYKNSKYHGVSLLNPPET LNL |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 350-432 of human Cyclin A2 (NP_001228.1). |
Additional Info
| Post Translational Modifications | Polyubiquitinated via 'Lys-11'-linked ubiquitin by the anaphase-promoting complex (APC/C), leading to its degradation by the proteasome. Deubiquitinated and stabilized by USP37 enables entry into S phase. Ubiquitinated during the G1 phase by the SCF(FBXO31) complex, leading to its proteasomal degradation. |
| Function | Cyclin which controls both the G1/S and the G2/M transition phases of the cell cycle. Functions through the formation of specific serine/threonine protein kinase holoenzyme complexes with the cyclin-dependent protein kinases CDK1 or CDK2. The cyclin subunit confers the substrate specificity of these complexes and differentially interacts with and activates CDK1 and CDK2 throughout the cell cycle. |
| Protein Name | Cyclin-A2Cyclin-ACyclin A |
| Database Links | Reactome: R-HSA-1362300Reactome: R-HSA-1538133Reactome: R-HSA-170145Reactome: R-HSA-171319Reactome: R-HSA-174184Reactome: R-HSA-176408Reactome: R-HSA-187577Reactome: R-HSA-2559582Reactome: R-HSA-2559586Reactome: R-HSA-5689880Reactome: R-HSA-5693607Reactome: R-HSA-6804116Reactome: R-HSA-6804756Reactome: R-HSA-6804757Reactome: R-HSA-68911Reactome: R-HSA-68949Reactome: R-HSA-69017Reactome: R-HSA-69273Reactome: R-HSA-69563Reactome: R-HSA-69656 |
| Cellular Localisation | NucleusCytoplasmExclusively Nuclear During InterphaseDetected In The Nucleus And The Cytoplasm At ProphaseCytoplasmic When Associated With Scaper |
| Alternative Antibody Names | Anti-Cyclin-A2 antibodyAnti-Cyclin-A antibodyAnti-Cyclin A antibodyAnti-CCNA2 antibodyAnti-CCN1 antibodyAnti-CCNA antibody |
Information sourced from Uniprot.org