• Western blot analysis of lysates from wild type (WT) and CBR1 knockout (KO) A-549 cells, using [KO Validated] CBR1 Rabbit polyclonal antibody (STJ11100047) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-CBR1 antibody (1-277) (STJ11100047)

SKU:
STJ11100047

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CBR1 (1-277) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CBR1
Gene ID: 873
Uniprot ID: CBR1_HUMAN
Immunogen Region: 1-277
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-277 of human CBR1 (NP_001748.1).
Immunogen Sequence: MSSGIHVALVTGGNKGIGLA IVRDLCRLFSGDVVLTARDV TRGQAAVQQLQAEGLSPRFH QLDIDDLQSIRALRDFLRKE YGGLDVLVNNAGIAFKVADP TPFHIQAEVTMKTNFFGTRD VCTELLPLIKPQGRVVNVSS IMSVRALKSCSPELQQKFRS ETITEEELVGLMNKFVEDTK KGVHQKEGWPSSAYGVTKIG VTVLSRIHARKLSEQRKGDK ILLNACCPGWVRTDMAGPK
Tissue Specificity Expressed in kidney (at protein level).
Function NADPH-dependent reductase with broad substrate specificity. Catalyzes the reduction of a wide variety of carbonyl compounds including quinones, prostaglandins, menadione, plus various xenobiotics. Catalyzes the reduction of the antitumor anthracyclines doxorubicin and daunorubicin to the cardiotoxic compounds doxorubicinol and daunorubicinol. Can convert prostaglandin E to prostaglandin F2-alpha. Can bind glutathione, which explains its higher affinity for glutathione-conjugated substrates. Catalyzes the reduction of S-nitrosoglutathione. In addition, participates in the glucocorticoid metabolism by catalyzing the NADPH-dependent cortisol/corticosterone into 20beta-dihydrocortisol (20b-DHF) or 20beta-corticosterone (20b-DHB), which are weak agonists of NR3C1 and NR3C2 in adipose tissue.
Protein Name Carbonyl Reductase Nadph 1
15-Hydroxyprostaglandin Dehydrogenase Nadp(+
20-Beta-Hydroxysteroid Dehydrogenase
Alcohol Dehydrogenase Nad(P+ Cbr1
Nadph-Dependent Carbonyl Reductase 1
Prostaglandin 9-Ketoreductase
Pg-9-Kr
Prostaglandin-E(2 9-Reductase
Short Chain Dehydrogenase/Reductase Family 21c Member 1
Database Links Reactome: R-HSA-2162123
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Carbonyl Reductase Nadph 1 antibody
Anti-15-Hydroxyprostaglandin Dehydrogenase Nadp(+ antibody
Anti-20-Beta-Hydroxysteroid Dehydrogenase antibody
Anti-Alcohol Dehydrogenase Nad(P+ Cbr1 antibody
Anti-Nadph-Dependent Carbonyl Reductase 1 antibody
Anti-Prostaglandin 9-Ketoreductase antibody
Anti-Pg-9-Kr antibody
Anti-Prostaglandin-E(2 9-Reductase antibody
Anti-Short Chain Dehydrogenase/Reductase Family 21c Member 1 antibody
Anti-CBR1 antibody
Anti-CBR antibody
Anti-CRN antibody
Anti-SDR21C1 antibody

Information sourced from Uniprot.org

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