![Western blot analysis of lysates from wild type (WT) and CAT1 knockdown (KD) HeLa cells using [KD Validated] CAT1 Rabbit pAb (STJ11107042) at1:1000 dilution. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 17 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 20s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/186238/407927/STJ11107042_1__86808.1744894391.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and CAT1 knockdown (KD) HeLa cells using [KD Validated] CAT1 Rabbit pAb (STJ11107042) at1:1000 dilution. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 17 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 20s.")
![Western blot analysis of lysates from Mouse heart using [KD Validated] CAT1 Rabbit pAb (STJ11107042) at1:1000 dilution. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time:20s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/186238/407928/STJ11107042_2__48893.1744894392.jpg?c=1 "Western blot analysis of lysates from Mouse heart using [KD Validated] CAT1 Rabbit pAb (STJ11107042) at1:1000 dilution. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time:20s.")
![Western blot analysis of lysates from wild type (WT) and CAT1 knockdown (KD) HeLa cells using [KD Validated] CAT1 Rabbit pAb (STJ11107042) at1:1000 dilution. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 17 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 20s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/186238/407927/STJ11107042_1__86808.1744894391.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and CAT1 knockdown (KD) HeLa cells using [KD Validated] CAT1 Rabbit pAb (STJ11107042) at1:1000 dilution. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 17 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 20s.")
Anti-CAT1 antibody (200-300 aa) (STJ11107042)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal CAT1 (200-300 aa) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SLC7A1 |
| Gene ID | 6541 |
| Uniprot ID | CTR1_HUMAN |
| Immunogen Region | 200-300 aa |
| Immunogen Sequence | VLGFIMVSGFVKGSVKNWQL TEEDFGNTSGRLCLNNDTKE GKPGVGGFMPFGFSGVLSGA ATCFYAFVGFDCIATTGEEV KNPQKAIPVGIVASLLICFI A |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 200-300 of human SLC7A1 (NP_003036.1). |
Additional Info
| Tissue Specificity | Ubiquitous. |
| Function | High-affinity, low capacity permease involved in the transport of the cationic amino acids (arginine, lysine and ornithine) in non-hepatic tissues. |
| Protein Name | High Affinity Cationic Amino Acid Transporter 1Cat-1Cat1Ecotropic Retroviral Leukemia Receptor HomologEcotropic Retrovirus Receptor HomologSolute Carrier Family 7 Member 1System Y+ Basic Amino Acid Transporter |
| Database Links | Reactome: R-HSA-352230 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-High Affinity Cationic Amino Acid Transporter 1 antibodyAnti-Cat-1 antibodyAnti-Cat1 antibodyAnti-Ecotropic Retroviral Leukemia Receptor Homolog antibodyAnti-Ecotropic Retrovirus Receptor Homolog antibodyAnti-Solute Carrier Family 7 Member 1 antibodyAnti-System Y+ Basic Amino Acid Transporter antibodyAnti-SLC7A1 antibodyAnti-ATRC1 antibodyAnti-ERR antibodyAnti-REC1L antibody |
Information sourced from Uniprot.org