• Immunohistochemistry analysis of CAST in paraffin-embedded human uterine cancer using CAST Rabbit polyclonal antibody (STJ119179) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of CAST in paraffin-embedded human stomach using CAST Rabbit polyclonal antibody (STJ119179) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using CAST Rabbit polyclonal antibody (STJ119179) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CAST antibody (1-350) (STJ119179)

SKU:
STJ119179

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CAST (1-350) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CAST
Gene ID: 831
Uniprot ID: ICAL_HUMAN
Immunogen Region: 1-350
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human CAST (NP_001177371.1).
Immunogen Sequence: MNPTETKAIPVSQQMEGPHL PNKKKHKKQAVKTEPEKKSQ STKLSVVHEKKSQEGKPKEH TEPKSLPKQASDTGSNDAHN KKAVSRSAEQQPSEKSTEPK TKPQDMISAGGESVAGITAI SGKPGDKKKEKKSLTPAVPV ESKPDKPSGKSGMDAALDDL IDTLGGPEETEEENTTYTGP EVSDPMSSTYIEELGKREVT IPPKYRELLAKPIGPDDAID ALSSDFTCGSPTAAGKKTE
Post Translational Modifications The N-terminus is blocked.
Function Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue.
Protein Name Calpastatin
Calpain Inhibitor
Sperm Bs-17 Component
Database Links Reactome: R-HSA-1474228
Reactome: R-HSA-8862803
Alternative Antibody Names Anti-Calpastatin antibody
Anti-Calpain Inhibitor antibody
Anti-Sperm Bs-17 Component antibody
Anti-CAST antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance