| Short Description : | Rabbit polyclonal CAMLG (1-190) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000IHC-P:1:50-1:100IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CAMLG |
| Gene ID: | 819 |
| Uniprot ID: | CAMLG_HUMAN |
| Immunogen Region: | 1-190 |
| Immunogen Sequence: | MESMAVATDGGERPGVPAGS GLSASQRRAELRRRKLLMNS EQRINRIMGFHRPGSGAEEE SQTKSKQQDSDKLNSLSVPS VSKRVVLGDSVSTGTTDQQG GVAEVKGTQLGDKLDSFIKP PECSSDVNLELRQRNRGDLT ADSVQRGSRHGLEQYLSRFE EAMKLRKQLISEKPSQEDGN TTEEFDSFRI |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human CAMLG (NP_001736.1). |
| Tissue Specificity | Ubiquitous. Highest levels in brain, testis and ovary. |
| Function | Required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Together with GET1/WRB, acts as a membrane receptor for soluble GET3/TRC40, which recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. Required for the stability of GET1. Stimulates calcium signaling in T cells through its involvement in elevation of intracellular calcium. Essential for the survival of peripheral follicular B cells. |
| Protein Name | Guided Entry Of Tail-Anchored Proteins Factor CamlgCalcium Signal-Modulating Cyclophilin Ligand |
| Database Links | Reactome: R-HSA-9609523 |
| Cellular Localisation | Endoplasmic Reticulum MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Guided Entry Of Tail-Anchored Proteins Factor Camlg antibodyAnti-Calcium Signal-Modulating Cyclophilin Ligand antibodyAnti-CAMLG antibodyAnti-CAML antibodyAnti-GET2 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and MYOF knockout (KO) HeLa (KO) cells, using [KO Validated] MYOF Rabbit polyclonal antibody (STJ117622) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/96581/379140/STJ117622_1__97810.1713146869.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and MYOF knockout (KO) HeLa (KO) cells, using [KO Validated] MYOF Rabbit polyclonal antibody (STJ117622) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
