• Immunofluorescence analysis of NIH/3T3 cells using CAMK2A Rabbit polyclonal antibody (STJ119903) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using CAMK2A Rabbit polyclonal antibody (STJ119903) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CAMK2A antibody (300-400) (STJ119903)

SKU:
STJ119903

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CAMK2A (300-400) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CAMK2A
Gene ID: 815
Uniprot ID: KCC2A_HUMAN
Immunogen Region: 300-400
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 300-400 of human CAMK2A (NP_741960.1).
Immunogen Sequence: KGAILTTMLATRNFSGGKSG GNKKSDGVKESSESTNTTIE DEDTKVRKQEIIKVTEQLIE AISNGDFESYTKMCDPGMTA FEPEALGNLVEGLDFHRFYF E
Post Translational Modifications Autophosphorylation of Thr-286 following activation by Ca(2+)/calmodulin. Phosphorylation of Thr-286 locks the kinase into an activated state. Palmitoylated. Probably palmitoylated by ZDHHC3 and ZDHHC7.
Function Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in various processes, such as synaptic plasticity, neurotransmitter release and long-term potentiation. Member of the NMDAR signaling complex in excitatory synapses, it regulates NMDAR-dependent potentiation of the AMPAR and therefore excitatory synaptic transmission. Regulates dendritic spine development. Also regulates the migration of developing neurons. Phosphorylates the transcription factor FOXO3 to activate its transcriptional activity. Phosphorylates the transcription factor ETS1 in response to calcium signaling, thereby decreasing ETS1 affinity for DNA. In response to interferon-gamma (IFN-gamma) stimulation, catalyzes phosphorylation of STAT1, stimulating the JAK-STAT signaling pathway. In response to interferon-beta (IFN-beta) stimulation, stimulates the JAK-STAT signaling pathway. Acts as a negative regulator of 2-arachidonoylglycerol (2-AG)-mediated synaptic signaling via modulation of DAGLA activity.
Protein Name Calcium/Calmodulin-Dependent Protein Kinase Type Ii Subunit Alpha
Cam Kinase Ii Subunit Alpha
Camk-Ii Subunit Alpha
Database Links Reactome: R-HSA-111932
Reactome: R-HSA-3371571
Reactome: R-HSA-399719
Reactome: R-HSA-4086398
Reactome: R-HSA-438066
Reactome: R-HSA-442982
Reactome: R-HSA-5576892
Reactome: R-HSA-5578775
Reactome: R-HSA-5673000
Reactome: R-HSA-5673001
Reactome: R-HSA-6802946
Reactome: R-HSA-6802952
Reactome: R-HSA-6802955
Reactome: R-HSA-877300
Reactome: R-HSA-9022692
Reactome: R-HSA-936837
Reactome: R-HSA-9609736
Reactome: R-HSA-9617324
Reactome: R-HSA-9620244
Reactome: R-HSA-9649948
Reactome: R-HSA-9656223
Cellular Localisation Synapse
Postsynaptic Density
Cell Projection
Dendritic Spine
Dendrite
Postsynaptic Lipid Rafts
Alternative Antibody Names Anti-Calcium/Calmodulin-Dependent Protein Kinase Type Ii Subunit Alpha antibody
Anti-Cam Kinase Ii Subunit Alpha antibody
Anti-Camk-Ii Subunit Alpha antibody
Anti-CAMK2A antibody
Anti-CAMKA antibody
Anti-KIAA0968 antibody

Information sourced from Uniprot.org

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