| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CALCOCO2 (1-446) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CALCOCO2 |
| Gene ID: |
10241 |
| Uniprot ID: |
CACO2_HUMAN |
| Immunogen Region: |
1-446 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-446 of human NDP52 (NP_005822.1). |
| Immunogen Sequence: |
MEETIKDPPTSAVLLDHCHF SQVIFNSVEKFYIPGGDVTC HYTFTQHFIPRRKDWIGIFR VGWKTTREYYTFMWVTLPID LNNKSAKQQEVQFKAYYLPK DDEYYQFCYVDEDGVVRGAS IPFQFRPENEEDILVVTTQG EVEEIEQHNKELCKENQELK DSCISLQKQNSDMQAELQKK QEELETLQSINKKLELKVKE QKDYWETELLQLKEQNQKMS SENEKMGIRVDQLQAQLST |
| Tissue Specificity | Expressed in all tissues tested with highest expression in skeletal muscle and lowest in brain. |
| Post Translational Modifications | (Microbial infection) Cleaved by S.pyogenes SpeB protease.leading to its degradation. Degradation by SpeB prevents autophagy, promoting to S.pyogenes intracellular replication. |
| Function | Xenophagy-specific receptor required for autophagy-mediated intracellular bacteria degradation. Acts as an effector protein of galectin-sensed membrane damage that restricts the proliferation of infecting pathogens such as Salmonella typhimurium upon entry into the cytosol by targeting LGALS8-associated bacteria for autophagy. Initially orchestrates bacteria targeting to autophagosomes and subsequently ensures pathogen degradation by regulating pathogen-containing autophagosome maturation. Bacteria targeting to autophagosomes relies on its interaction with MAP1LC3A, MAP1LC3B and/or GABARAPL2, whereas regulation of pathogen-containing autophagosome maturation requires the interaction with MAP3LC3C. May play a role in ruffle formation and actin cytoskeleton organization and seems to negatively regulate constitutive secretion. |
| Protein Name | Calcium-Binding And Coiled-Coil Domain-Containing Protein 2Antigen Nuclear Dot 52 Kda ProteinNuclear Domain 10 Protein Ndp52Nuclear Domain 10 Protein 52Nuclear Dot Protein 52 |
| Cellular Localisation | CytoplasmPerinuclear RegionCytoskeletonCytoplasmic VesicleAutophagosome MembranePeripheral Membrane ProteinLocalizes To Nuclear DotsIt Is Not A Nuclear Dot-Associated Protein But Localizes Predominantly In The Cytoplasm With A Coarse-Grained Distribution Preferentially Close To The Nucleus |
| Alternative Antibody Names | Anti-Calcium-Binding And Coiled-Coil Domain-Containing Protein 2 antibodyAnti-Antigen Nuclear Dot 52 Kda Protein antibodyAnti-Nuclear Domain 10 Protein Ndp52 antibodyAnti-Nuclear Domain 10 Protein 52 antibodyAnti-Nuclear Dot Protein 52 antibodyAnti-CALCOCO2 antibodyAnti-NDP52 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
