Anti-CALCOCO2 antibody (1-446) (STJ29497)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal CALCOCO2 (1-446) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CALCOCO2 |
| Gene ID | 10241 |
| Uniprot ID | CACO2_HUMAN |
| Immunogen Region | 1-446 |
| Immunogen Sequence | MEETIKDPPTSAVLLDHCHF SQVIFNSVEKFYIPGGDVTC HYTFTQHFIPRRKDWIGIFR VGWKTTREYYTFMWVTLPID LNNKSAKQQEVQFKAYYLPK DDEYYQFCYVDEDGVVRGAS IPFQFRPENEEDILVVTTQG EVEEIEQHNKELCKENQELK DSCISLQKQNSDMQAELQKK QEELETLQSINKKLELKVKE QKDYWETELLQLKEQNQKMS SENEKMGIRVDQLQAQLST |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-283 of human NDP52 (NP_005822.1). |
Additional Info
| Tissue Specificity | Expressed in all tissues tested with highest expression in skeletal muscle and lowest in brain. |
| Post Translational Modifications | (Microbial infection) Cleaved by S.pyogenes SpeB protease.leading to its degradation. Degradation by SpeB prevents autophagy, promoting to S.pyogenes intracellular replication. |
| Function | Xenophagy-specific receptor required for autophagy-mediated intracellular bacteria degradation. Acts as an effector protein of galectin-sensed membrane damage that restricts the proliferation of infecting pathogens such as Salmonella typhimurium upon entry into the cytosol by targeting LGALS8-associated bacteria for autophagy. Initially orchestrates bacteria targeting to autophagosomes and subsequently ensures pathogen degradation by regulating pathogen-containing autophagosome maturation. Bacteria targeting to autophagosomes relies on its interaction with MAP1LC3A, MAP1LC3B and/or GABARAPL2, whereas regulation of pathogen-containing autophagosome maturation requires the interaction with MAP3LC3C. May play a role in ruffle formation and actin cytoskeleton organization and seems to negatively regulate constitutive secretion. |
| Protein Name | Calcium-Binding And Coiled-Coil Domain-Containing Protein 2Antigen Nuclear Dot 52 Kda ProteinNuclear Domain 10 Protein Ndp52Nuclear Domain 10 Protein 52Nuclear Dot Protein 52 |
| Database Links | |
| Cellular Localisation | CytoplasmPerinuclear RegionCytoskeletonCytoplasmic VesicleAutophagosome MembranePeripheral Membrane ProteinLocalizes To Nuclear DotsIt Is Not A Nuclear Dot-Associated Protein But Localizes Predominantly In The Cytoplasm With A Coarse-Grained Distribution Preferentially Close To The Nucleus |
| Alternative Antibody Names | Anti-Calcium-Binding And Coiled-Coil Domain-Containing Protein 2 antibodyAnti-Antigen Nuclear Dot 52 Kda Protein antibodyAnti-Nuclear Domain 10 Protein Ndp52 antibodyAnti-Nuclear Domain 10 Protein 52 antibodyAnti-Nuclear Dot Protein 52 antibodyAnti-CALCOCO2 antibodyAnti-NDP52 antibody |
Information sourced from Uniprot.org