• Immunofluorescence analysis of L929 cells using CA5B antibody (STJ119691) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates, using CA5B Rabbit polyclonal antibody (STJ119691) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CA5B antibody (1-317) (STJ119691)

SKU:
STJ119691

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CA5B (1-317) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CA5B
Gene ID: 11238
Uniprot ID: CAH5B_HUMAN
Immunogen Region: 1-317
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-317 of human CA5B (NP_009151.1).
Immunogen Sequence: MVVMNSLRVILQASPGKLLW RKFQIPRFMPARPCSLYTCT YKTRNRALHPLWESVDLVPG GDRQSPINIRWRDSVYDPGL KPLTISYDPATCLHVWNNGY SFLVEFEDSTDKSVIKGGPL EHNYRLKQFHFHWGAIDAWG SEHTVDSKCFPAELHLVHWN AVRFENFEDAALEENGLAVI GVFLKLGKHHKELQKLVDTL PSIKHKDALVEFGSFDPSCL MPTCPDYWTYSGSLTTPPL
Tissue Specificity Strongest expression in heart, pancreas, kidney, placenta, lung, and skeletal muscle. Not expressed in liver.
Function Reversible hydration of carbon dioxide.
Protein Name Carbonic Anhydrase 5b - Mitochondrial
Carbonate Dehydratase Vb
Carbonic Anhydrase Vb
Ca-Vb
Database Links Reactome: R-HSA-1475029
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Carbonic Anhydrase 5b - Mitochondrial antibody
Anti-Carbonate Dehydratase Vb antibody
Anti-Carbonic Anhydrase Vb antibody
Anti-Ca-Vb antibody
Anti-CA5B antibody

Information sourced from Uniprot.org

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