| Short Description : | Rabbit polyclonal C7 (22-270) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | C7 |
| Gene ID: | 730 |
| Uniprot ID: | CO7_HUMAN |
| Immunogen Region: | 22-270 |
| Immunogen Sequence: | ASSPVNCQWDFYAPWSECNG CTKTQTRRRSVAVYGQYGGQ PCVGNAFETQSCEPTRGCPT EEGCGERFRCFSGQCISKSL VCNGDSDCDEDSADEDRCED SERRPSCDIDKPPPNIELTG NGYNELTGQFRNRVINTKSF GGQCRKVFSGDGKDFYRLSG NVLSYTFQVKINNDFNYEFY NSTWSYVKHTSTEHTSSSRK RSFFRSSSSSSRSYTSHTNE IHKGKSYQLLVVENTVEVA |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 22-270 of human C7 (NP_000578.2). |
| Post Translational Modifications | C-, N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. |
| Function | Component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane rupture and cell lysis. The MAC is initiated by proteolytic cleavage of C5 into complement C5b in response to the classical, alternative, lectin and GZMK complement pathways. The complement pathways consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. C7 serves as a membrane anchor. During MAC assembly, associates with C5b and C6 to form the C5b-7 complex, a key lipophilic precursor of the MAC complex, which associates with the outer leaflet and reduces the energy for membrane bending. |
| Protein Name | Complement Component C7 |
| Database Links | Reactome: R-HSA-166665Reactome: R-HSA-977606 |
| Cellular Localisation | SecretedTarget Cell MembraneSecreted As Soluble ProteinInserts Into The Cell Membrane Of Target Cells |
| Alternative Antibody Names | Anti-Complement Component C7 antibodyAnti-C7 antibody |
Information sourced from Uniprot.org
