• Western blot analysis of lysates from HepG2 cells, using C1S Rabbit polyclonal antibody (STJ28958) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-C1S antibody (479-688) (STJ28958)

SKU:
STJ28958

£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: C1S
Gene ID: 716
Uniprot ID: C1S_HUMAN
Immunogen Region: 479-688
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 479-688 of human C1S (NP_958850.1).
Immunogen Sequence: GNREPTMYVGSTSVQTSRLA KSKMLTPEHVFIHPGWKLLE VPEGRTNFDNDIALVRLKDP VKMGPTVSPICLPGTSSDYN LMDGDLGLISGWGRTEKRDR AVRLKAARLPVAPLRKCKEV KVEKPTADAEAYVFTPNMIC AGGEKGMDSCKGDSGGAFAV QDPNDKTKFYAAGLVSWGPQ CGTYGLYTRVKNYVDWIMKT MQENSTPRED
Post Translational Modifications The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
Function C1s B chain is a serine protease that combines with C1q and C1r to form C1, the first component of the classical pathway of the complement system. C1r activates C1s so that it can, in turn, activate C2 and C4. Cleaves also IGFBP5 and thereby inhibits the trophic effects of IGF1.
Protein Name Complement C1s Subcomponent
C1 Esterase
Complement Component 1 Subcomponent S Cleaved Into - Complement C1s Subcomponent Heavy Chain - Complement C1s Subcomponent Light Chain
Database Links Reactome: R-HSA-166663
Reactome: R-HSA-173623
Reactome: R-HSA-977606
Alternative Antibody Names Anti-Complement C1s Subcomponent antibody
Anti-C1 Esterase antibody
Anti-Complement Component 1 Subcomponent S Cleaved Into - Complement C1s Subcomponent Heavy Chain - Complement C1s Subcomponent Light Chain antibody
Anti-C1S antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance