Anti-C1S antibody (479-688) (STJ28958)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal C1S (479-688) antibody for use in WB and ELISA in human and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | C1S |
| Gene ID | 716 |
| Uniprot ID | C1S_HUMAN |
| Immunogen Region | 479-688 |
| Immunogen Sequence | GNREPTMYVGSTSVQTSRLA KSKMLTPEHVFIHPGWKLLE VPEGRTNFDNDIALVRLKDP VKMGPTVSPICLPGTSSDYN LMDGDLGLISGWGRTEKRDR AVRLKAARLPVAPLRKCKEV KVEKPTADAEAYVFTPNMIC AGGEKGMDSCKGDSGGAFAV QDPNDKTKFYAAGLVSWGPQ CGTYGLYTRVKNYVDWIMKT MQENSTPRED |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 479-688 of human C1S (NP_958850.1). |
Additional Info
| Post Translational Modifications | Cleaved and activated by C1R to generate Complement C1s subcomponent heavy and light chains. The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. |
| Function | Component of the complement C1 complex, a multiprotein complex that initiates the classical pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. C1S is activated following association of the C1 complex with immunoglobulins (IgG or IgM) complexed with antigens to form antigen-antibody complexes on the surface of pathogens. C1S is cleaved and activated by C1R to generate C1s subcomponent heavy and light chains. C1s subcomponent light chain then cleaves and activates C2 and C4, the next components of the classical complement pathway. Complement C1s subcomponent light chain: Serine protease component of the complement C1 complex, which catalyzes cleavage and activation of C2 and C4, the next components of the classical complement pathway. Also able to cleave C1 inhibitor (SERPING1) in vitro.additional evidence is however required to confirm this result in vivo. Also cleaves IGFBP5 and thereby inhibits the trophic effects of IGF1. |
| Protein Name | Complement C1s SubcomponentC1 EsteraseComplement Component 1 Subcomponent S Cleaved Into - Complement C1s Subcomponent Heavy ChainComplement C1s Subcomponent Chain A - Complement C1s Subcomponent Light ChainComplement C1s Subcomponent Chain B |
| Database Links | Reactome: R-HSA-166663Reactome: R-HSA-173623Reactome: R-HSA-977606 |
| Cellular Localisation | SecretedCell SurfaceRecruited To The Surface Of Pathogens By The C1q Subcomplex |
| Alternative Antibody Names | Anti-Complement C1s Subcomponent antibodyAnti-C1 Esterase antibodyAnti-Complement Component 1 Subcomponent S Cleaved Into - Complement C1s Subcomponent Heavy Chain antibodyAnti-Complement C1s Subcomponent Chain A - Complement C1s Subcomponent Light Chain antibodyAnti-Complement C1s Subcomponent Chain B antibodyAnti-C1S antibody |
Information sourced from Uniprot.org