• Immunofluorescence analysis of PC-12 cells using C1qa Rabbit polyclonal antibody (STJ11105385) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using C1qa Rabbit polyclonal antibody (STJ11105385) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of C1qa in paraffin-embedded Human tonsil tissue using C1qa Rabbit polyclonal antibody (STJ11105385) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using C1qa Rabbit polyclonal antibody (STJ11105385) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-C1qa antibody (23-245) (STJ11105385)

SKU:
STJ11105385

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-C1qa (23-245) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:500-1:1000
IF/ICC 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: C1qa
Gene ID: 12259
Uniprot ID: C1QA_MOUSE
Immunogen Region: 23-245
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 23-245 of mouse C1qa (NP_031598.2).
Immunogen Sequence: EDVCRAPNGKDGAPGNPGRP GRPGLKGERGEPGAAGIRTG IRGFKGDPGESGPPGKPGNV GLPGPSGPLGDSGPQGLKGV KGNPGNIRDQPRPAFSAIRQ NPMTLGNVVIFDKVLTNQES PYQNHTGRFICAVPGFYYFN FQVISKWDLCLFIKSSSGGQ PRDSLSFSNTNNKGLFQVLA GGTVLQLRRGDEVWIEKDPA KGRIYQGTEADSIFSGFLIF PSA
Post Translational Modifications O-linked glycans are Glc-Gal disaccharides typically found as secondary modifications of hydroxylated lysines in collagen-like domains. Proline residues in the collagen-like domain motif, GXPG, are typically 4-hydroxylated.
Function C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
Protein Name Complement C1q Subcomponent Subunit A
Database Links Reactome: R-MMU-166663
Reactome: -MMU-173623
Reactome: -MMU-977606
Cellular Localisation Secreted
Alternative Antibody Names Anti-Complement C1q Subcomponent Subunit A antibody
Anti-C1qa antibody

Information sourced from Uniprot.org

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