• Western blot detection of BRAP in A549 and HUVEC cell lysates using BRAP mouse mAb (1:500 diluted).Predicted band size:67KDa.Observed band size:67KDa.
  • Western blot analysis of extracts from CHO-K1 cells, transfected with a human pFLAG-CMV2-BRAP construct (A) or transfected with a human pFLAG-CMV2 construct (B) , using BRAP mouse mAb (1:1000 diluted).

Anti-BRAP antibody [1E7-C9-D10-B10] (STJ99215)

SKU:
STJ99215

£139.00 - £336.00
Current Stock:
Host: Mouse
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-BRCA1-associated protein is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: 1E7-C9-D10-B10
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BRAP
Gene ID: 8315
Uniprot ID: BRAP_HUMAN
Specificity: This antibody detects endogenous levels of BRAP and does not cross-react with related proteins.
Immunogen: Purified recombinant human BRAP protein fragments expressed in E.coli.
Function Negatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. Also acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto-polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation. May also act as a cytoplasmic retention protein with a role in regulating nuclear transport.
Protein Name Brca1-Associated Protein
Brap2
Impedes Mitogenic Signal Propagation
Imp
Ring Finger Protein 52
Ring-Type E3 Ubiquitin Transferase Brap2
Renal Carcinoma Antigen Ny-Ren-63
Database Links Reactome: R-HSA-5673000
Reactome: R-HSA-5675221
Reactome: R-HSA-6802946
Reactome: R-HSA-6802955
Reactome: R-HSA-9649948
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Brca1-Associated Protein antibody
Anti-Brap2 antibody
Anti-Impedes Mitogenic Signal Propagation antibody
Anti-Imp antibody
Anti-Ring Finger Protein 52 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Brap2 antibody
Anti-Renal Carcinoma Antigen Ny-Ren-63 antibody
Anti-BRAP antibody
Anti-RNF52 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance