Anti-BRAP antibody (1-200) (STJ118879)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal BRAP (1-200) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | BRAP |
| Gene ID | 8315 |
| Uniprot ID | BRAP_HUMAN |
| Immunogen Region | 1-200 |
| Immunogen Sequence | MSVSLVVIRLELAEHSPVPA GFGFSAAAGEMSDEEIKKTT LASAVACLEGKSPGEKVAII HQHLGRREMTDVIIETMKSN PDELKTTVEERKSSEASPTA QRSKDHSKECINAAPDSPSK QLPDQISFFSGNPSVEIVHG IMHLYKTNKMTSLKEDVRRS AMLCILTVPAAMTSHDLMKF VAPFNEVIEQMKIIRDSTPN |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human BRAP (NP_006759.3). |
Additional Info
| Tissue Specificity | Expressed in breast epithelial cell lines. |
| Function | Negatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. Also acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto-polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation. May also act as a cytoplasmic retention protein with a role in regulating nuclear transport. |
| Protein Name | Brca1-Associated ProteinBrap2Impedes Mitogenic Signal PropagationImpRing Finger Protein 52Ring-Type E3 Ubiquitin Transferase Brap2Renal Carcinoma Antigen Ny-Ren-63 |
| Database Links | Reactome: R-HSA-5673000Reactome: R-HSA-5675221Reactome: R-HSA-6802946Reactome: R-HSA-6802955Reactome: R-HSA-9649948 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Brca1-Associated Protein antibodyAnti-Brap2 antibodyAnti-Impedes Mitogenic Signal Propagation antibodyAnti-Imp antibodyAnti-Ring Finger Protein 52 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Brap2 antibodyAnti-Renal Carcinoma Antigen Ny-Ren-63 antibodyAnti-BRAP antibodyAnti-RNF52 antibody |
Information sourced from Uniprot.org