Anti-BMP4 antibody (Internal) (STJ73557)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-BMP4 (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 40-45kDa band observed in lysates of cell lines A431 and HeLa (calculated MW of 46.6kDa according to NP_001193.2). Recommended concentration: 0.3-1µg/ml. |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | BMP4 |
| Gene ID | 652 |
| Uniprot ID | BMP4_HUMAN |
| Accession Number | NP_001193.2 |
| Immunogen Region | Internal |
| Immunogen Sequence | SPKHHSQRARK |
| Specificity | The immunizing peptide represents the N terminus of the mature protein. Reported variants represent identical protein: NP_570911.2, NP_001193.2, NP_570912.2. |
Additional Info
| Function | Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes, including neurogenesis, vascular development, angiogenesis and osteogenesis. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction. Initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2. Once all three components are bound together in a complex at the cell surface, BMPR2 phosphorylates and activates BMPR1A. In turn, BMPR1A propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes. Positively regulates the expression of odontogenic development regulator MSX1 via inducing the IPO7-mediated import of SMAD1 to the nucleus. Required for MSX1-mediated mesenchymal molar tooth bud development beyond the bud stage, via promoting Wnt signaling. Acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, expression is repressed during the bell stage by MSX1-mediated inhibition of CTNNB1 signaling. Able to induce its own expression in dental mesenchymal cells and also in the neighboring dental epithelial cells via an MSX1-mediated pathway. Can also signal through non-canonical BMP pathways such as ERK/MAP kinase, PI3K/Akt, or SRC cascades. For example, induces SRC phosphorylation which, in turn, activates VEGFR2, leading to an angiogenic response. |
| Protein Name | Bone Morphogenetic Protein 4Bmp-4Bone Morphogenetic Protein 2bBmp-2b |
| Database Links | Reactome: R-HSA-2129379Reactome: R-HSA-381426Reactome: R-HSA-8957275Reactome: R-HSA-9754189Reactome: R-HSA-9758920Reactome: R-HSA-9761174Reactome: R-HSA-9793380Reactome: R-HSA-9827857Reactome: R-HSA-9830364Reactome: R-HSA-9830674Reactome: R-HSA-9834899 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular Matrix |
| Alternative Antibody Names | Anti-Bone Morphogenetic Protein 4 antibodyAnti-Bmp-4 antibodyAnti-Bone Morphogenetic Protein 2b antibodyAnti-Bmp-2b antibodyAnti-BMP4 antibodyAnti-BMP2B antibodyAnti-DVR4 antibody |
Information sourced from Uniprot.org