• Western blot analysis of extracts of various cell lines, using BFAR antibody (STJ110606) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of U-2 OS cells using BFAR Polyclonal Antibody (STJ110606) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using BFAR Polyclonal Antibody (STJ110606) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using BFAR Polyclonal Antibody (STJ110606) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using BFAR Polyclonal Antibody (STJ110606) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using BFAR Polyclonal Antibody (STJ110606) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-2 OS cells using BFAR Polyclonal Antibody (STJ110606) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using BFAR antibody (STJ110606) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-BFAR antibody (1-140) (STJ110606)

SKU:
STJ110606

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BFAR (1-140) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BFAR
Gene ID: 51283
Uniprot ID: BFAR_HUMAN
Immunogen Region: 1-140
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human BFAR (NP_057645.1).
Immunogen Sequence: MEEPQKSYVNTMDLERDEPL KSTGPQISVSEFSCHCCYDI LVNPTTLNCGHSFCRHCLAL WWASSKKTECPECREKWEGF PKVSILLRDAIEKLFPDAIR LRFEDIQQNNDIVQSLAAFQ KYGNDQIPLAPNTGRANQQM
Tissue Specificity Expressed highly in brain, moderately in small intestine, weakly in testes and only faintly in liver and skeletal muscle. Not expressed in heart, kidney, lung and spleen.
Function Apoptosis regulator. Has anti-apoptotic activity, both for apoptosis triggered via death-receptors and via mitochondrial factors.
Protein Name Bifunctional Apoptosis Regulator
Ring Finger Protein 47
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Bifunctional Apoptosis Regulator antibody
Anti-Ring Finger Protein 47 antibody
Anti-BFAR antibody
Anti-BAR antibody
Anti-RNF47 antibody

Information sourced from Uniprot.org

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