HeLa lysate either in RIPA buffer (lanes 1, 2, 4, 6) or TX100 buffer (lanes 3, 5, 7) overexpressing Mouse ßCstf-64 (DYKDDDDK tagged) immunoprecipitated using STJ72911 (2ug) with Protein G-coated magnetic beads in lanes 6 and 7 and probed with anti-DYKDDDDK (1/1000). Mock-transfected HeLa in Lane 1. A 1/40th part of the lysate was loaded in lanes 2 and 3 before IP and in lanes 4 an 5 after IP. Primary incubations were for 1 hour. Detected by chemiluminescence. Data obtained from Dr P Grozdanov, Texas Tech University Health Sciences Center, USA.

Anti-betaCstF-64 variant 1/betaCstF-64 variant 3-Mouse antibody (Internal) (STJ72911)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
STJ72911-100
🚚 Free UK Delivery on orders over £150
Processing The item has been added
Enquire For Bulk Order
✓ 1-Year Performance Guarantee | SDS & Datasheet Included | Expert Tech Support | Covered by St John's Laboratory Guarantee

General Information

Short DescriptionGoat polyclonal antibody anti-betaCstF-64 variant 1/betaCstF-64 variant 3-Mouse (Internal) is suitable for use in ELISA, Immunohistochemistry and Immunoprecipitation research applications.
ApplicationsPep-ELISA/IHC/IP
HostGoat
ReactivityMouse
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration0.5 mg/mL
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution RangePeptide ELISA: antibody detection limit dilution 1:128000.
WB: IP: In transfected HeLa transiently expressing Mouse ßCstf-64 ( (DYKDDDDK tagged) , a band of approx. 80kDa was observed using anti-DYKDDDDK antibody in Western blot after immunopreci
Formulation0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage InstructionStore at-20Β°C on receipt and minimise freeze-thaw cycles.

Target Information

Accession NumberACF05702.1; ACF05700.1
Immunogen RegionInternal
Immunogen SequencePASIERVQGQRT
SpecificityThis antibody recognizes mouse variants 1 and 3 of the reported beta isoform (ACF05702.1; ACF05700.1).

Additional Info

Function One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. This subunit is directly involved in the binding to pre-mRNAs.
Protein Name Cleavage Stimulation Factor Subunit 2
Cf-1 64 Kda Subunit
Cleavage Stimulation Factor 64 Kda Subunit
Cstf 64 Kda Subunit
Cstf-64
Database Links Reactome: R-MMU-72163
Reactome: -MMU-72187
Reactome: -MMU-73856
Reactome: -MMU-77595
Cellular Localisation Nucleus
Localized With Ddx1 In Cleavage Bodies
Alternative Antibody Names Anti-Cleavage Stimulation Factor Subunit 2 antibody
Anti-Cf-1 64 Kda Subunit antibody
Anti-Cleavage Stimulation Factor 64 Kda Subunit antibody
Anti-Cstf 64 Kda Subunit antibody
Anti-Cstf-64 antibody
Anti-Cstf2 antibody

Information sourced from Uniprot.org

Citations

Product Review

!