![Western blot analysis of various lysates using [KD Validated] Bcl9 Rabbit pAb (STJ28878) at 1:1000 dilution. <br/> Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/> Lysates/ proteins: 25 Mu g per lane. <br/> Blocking buffer: 3 % nonfat dry milk in TBST. <br/> Detection: ECL Basic Kit <br/> Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/102127/411415/STJ28878_2__50460.1744897941.jpg?c=1 "Western blot analysis of various lysates using [KD Validated] Bcl9 Rabbit pAb (STJ28878) at 1:1000 dilution. <br/> Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/> Lysates/ proteins: 25 Mu g per lane. <br/> Blocking buffer: 3 % nonfat dry milk in TBST. <br/> Detection: ECL Basic Kit <br/> Exposure time: 60s.")
![Western blot analysis of various lysates using [KD Validated] Bcl9 Rabbit pAb (STJ28878) at 1:1000 dilution. <br> Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br> Lysates/ proteins: 25 Mu g per lane. <br> Blocking buffer: 3 % nonfat dry milk in TBST. <br> Detection: ECL Basic Kit <br> Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/102127/411419/STJ28878_2__92022.1744897942.jpg?c=1 "Western blot analysis of various lysates using [KD Validated] Bcl9 Rabbit pAb (STJ28878) at 1:1000 dilution. <br> Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br> Lysates/ proteins: 25 Mu g per lane. <br> Blocking buffer: 3 % nonfat dry milk in TBST. <br> Detection: ECL Basic Kit <br> Exposure time: 60s.")
![Western blot analysis of lysates from wild type (WT) and Bcl9 knockdown (KD) 293T cells using [KD Validated] Bcl9 Rabbit pAb (STJ28878) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/102127/411416/STJ28878_3__70506.1744897941.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Bcl9 knockdown (KD) 293T cells using [KD Validated] Bcl9 Rabbit pAb (STJ28878) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.")
![Western blot analysis of lysates from wild type (WT) and Bcl9 knockdown (KD) 293T cells using [KD Validated] Bcl9 Rabbit pAb (STJ28878) at1:1000 dilution. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/102127/411420/STJ28878_3__19057.1744897943.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Bcl9 knockdown (KD) 293T cells using [KD Validated] Bcl9 Rabbit pAb (STJ28878) at1:1000 dilution. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 60s.")
Anti-BCL9 antibody (STJ28878)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Bcl9 antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | Bcl9 |
| Gene ID | 77578 |
| Uniprot ID | BCL9_MOUSE |
| Specificity | Recombinant protein of mouse Bcl9 |
Additional Info
| Function | Promotes beta-catenin's transcriptional activity. Involved in signal transduction through the Wnt pathway. |
| Protein Name | B-Cell Cll/Lymphoma 9 ProteinB-Cell Lymphoma 9 ProteinBcl-9 |
| Database Links | Reactome: R-MMU-201722 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-B-Cell Cll/Lymphoma 9 Protein antibodyAnti-B-Cell Lymphoma 9 Protein antibodyAnti-Bcl-9 antibodyAnti-Bcl9 antibody |
Information sourced from Uniprot.org