Anti-BCL3 antibody (300-400) (STJ113187)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal BCL3 (300-400) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | BCL3 |
| Gene ID | 602 |
| Uniprot ID | BCL3_HUMAN |
| Immunogen Region | 300-400 |
| Immunogen Sequence | ANVNAQMYSGSSALHSASGR GLLPLVRTLVRSGADSSLKN CHNDTPLMVARSRRVIDILR GKATRPASTSQPDPSPDRSA NTSPESSSRLSSNGLLSASP S |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 300-400 of human BCL3 (NP_005169.2). |
Additional Info
| Post Translational Modifications | Polyubiquitinated. Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains. Deubiquitination by CYLD prevents nuclear accumulation. Activated by phosphorylation. |
| Function | Contributes to the regulation of transcriptional activation of NF-kappa-B target genes. In the cytoplasm, inhibits the nuclear translocation of the NF-kappa-B p50 subunit. In the nucleus, acts as transcriptional activator that promotes transcription of NF-kappa-B target genes. Contributes to the regulation of cell proliferation. |
| Protein Name | B-Cell Lymphoma 3 ProteinBcl-3Proto-Oncogene Bcl3 |
| Database Links | |
| Cellular Localisation | NucleusCytoplasmPerinuclear RegionUbiquitination Via 'Lys-63'-Linked Ubiquitin Chains Is Required For Nuclear Accumulation |
| Alternative Antibody Names | Anti-B-Cell Lymphoma 3 Protein antibodyAnti-Bcl-3 antibodyAnti-Proto-Oncogene Bcl3 antibodyAnti-BCL3 antibodyAnti-BCL4 antibodyAnti-D19S37 antibody |
Information sourced from Uniprot.org