| Short Description : | Rabbit polyclonal BCL3 (300-400) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | BCL3 |
| Gene ID: | 602 |
| Uniprot ID: | BCL3_HUMAN |
| Immunogen Region: | 300-400 |
| Immunogen Sequence: | ANVNAQMYSGSSALHSASGR GLLPLVRTLVRSGADSSLKN CHNDTPLMVARSRRVIDILR GKATRPASTSQPDPSPDRSA NTSPESSSRLSSNGLLSASP S |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 300-400 of human BCL3 (NP_005169.2). |
| Post Translational Modifications | Polyubiquitinated. Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains. Deubiquitination by CYLD prevents nuclear accumulation. Activated by phosphorylation. |
| Function | Contributes to the regulation of transcriptional activation of NF-kappa-B target genes. In the cytoplasm, inhibits the nuclear translocation of the NF-kappa-B p50 subunit. In the nucleus, acts as transcriptional activator that promotes transcription of NF-kappa-B target genes. Contributes to the regulation of cell proliferation. |
| Protein Name | B-Cell Lymphoma 3 ProteinBcl-3Proto-Oncogene Bcl3 |
| Database Links | |
| Cellular Localisation | NucleusCytoplasmPerinuclear RegionUbiquitination Via 'Lys-63'-Linked Ubiquitin Chains Is Required For Nuclear Accumulation |
| Alternative Antibody Names | Anti-B-Cell Lymphoma 3 Protein antibodyAnti-Bcl-3 antibodyAnti-Proto-Oncogene Bcl3 antibodyAnti-BCL3 antibodyAnti-BCL4 antibodyAnti-D19S37 antibody |
Information sourced from Uniprot.org
