Anti-BCDIN3D antibody (1-292) (STJ119854)

SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ119854
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General Information

Short DescriptionRabbit polyclonal BCDIN3D (1-292) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman/Mouse
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolBCDIN3D
Gene ID144233
Uniprot IDBN3D2_HUMAN
Immunogen Region1-292
Immunogen SequenceMAVPTELDGGSVKETAAEEE SRVLAPGAAPFGNFPHYSRF HPPEQRLRLLPPELLRQLFP ESPENGPILGLDVGCNSGDL SVALYKHFLSLPDGETCSDA SREFRLLCCDIDPVLVKRAE KECPFPDALTFITLDFMNQR TRKVLLSSFLSQFGRSVFDI GFCMSITMWIHLNHGDHGLW EFLAHLSSLCHYLLVEPQPW KCYRAAARRLRKLGLHDFDH FHSLAIRGDMPNQIVQILT
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 1-292 of human BCDIN3D (NP_859059.1).

Additional Info

Function O-methyltransferase that specifically monomethylates 5'-monophosphate of cytoplasmic histidyl tRNA (tRNA(His)), acting as a capping enzyme by protecting tRNA(His) from cleavage by DICER1. Also able, with less efficiently, to methylate the 5' monophosphate of a subset of pre-miRNAs, acting as a negative regulator of miRNA processing. The 5' monophosphate of pre-miRNAs is recognized by DICER1 and is required for pre-miRNAs processing: methylation at this position reduces the processing of pre-miRNAs by DICER1. Was also reported to mediate dimethylation of pre-miR-145.however dimethylation cannot be reproduced by another group which observes a monomethylation of pre-miR-145.
Protein Name Rna 5'-Monophosphate Methyltransferase
Bcdin3 Domain-Containing Protein
Database Links Reactome: R-HSA-203927
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Rna 5'-Monophosphate Methyltransferase antibody
Anti-Bcdin3 Domain-Containing Protein antibody
Anti-BCDIN3D antibody

Information sourced from Uniprot.org

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