• Confocal imaging of U-2 OS cells using PUMA Rabbit monoclonal antibody (STJ11101945, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
  • Confocal imaging of NIH/3T3 cells using PUMA Rabbit monoclonal antibody (STJ11101945, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
  • Immunohistochemistry analysis of PUMA in paraffin-embedded mouse spinal cord using PUMA Rabbit monoclonal antibody (STJ11101945) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of PUMA in paraffin-embedded rat brain using PUMA Rabbit monoclonal antibody (STJ11101945) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using PUMA Rabbit monoclonal antibody (STJ11101945) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-BBC3 antibody (100-193) [S5MR] (STJ11101945)

SKU:
STJ11101945

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-PUMA (100-193) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BBC3
BBC3
Gene ID: 27113
27113
Uniprot ID: BBC3_HUMAN
BBC3B_HUMAN
Immunogen Region: 100-193
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-193 of human PUMA (NP_055232.1)
Immunogen Sequence: AEQHLESPVPSAPGALAGGP TQAAPGVRGEEEQWAREIGA QLRRMADDLNAQYERRRQEE QQRHRPSPWRVLYNLIMGLL PLPRGHRAPEMEPN
Tissue Specificity
Alternative Antibody Names Anti-Bcl-2-Binding Component 3 - Isoforms 1/2 antibody
Anti-Jfy-1 antibody
Anti-P53 Up-Regulated Modulator Of Apoptosis antibody
Anti-BBC3 antibody
Anti-PUMA antibody
Anti-Bcl-2-Binding Component 3 - Isoforms 3/4 antibody
Anti-Jfy-1 antibody
Anti-P53 Up-Regulated Modulator Of Apoptosis antibody
Anti-BBC3 antibody
Anti-PUMA antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance