• STJ70537 (2µg/ml) staining of Rat Skeletal Muscle lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70537 (0. 5µg/ml) staining of NIH3T3 nuclear cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70537 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70537 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70537 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-BAF53A and BAF53B antibody (C-Term) (STJ70537)

SKU:
STJ70537-100

€390.63
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Host: Goat
Applications: Pep-ELISA/WB/IF/FC
Reactivity: Human/Mouse/Rat/Dog/Pig/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-BAF53A and BAF53B (C-Term) is suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:16000.
WB: Approx 48kDa band observed in Rat Skeletal Muscle lysates and approx. 50kDa in nuclear lysates of cell line NIH3T3 and in preliminary testing of Human Colorectal cancer and Mouse Skele
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: ACTL6B
Gene ID: 51412
Uniprot ID: ACL6B_HUMAN
Immunogen Region: C-Term
Accession Number: NP_004292.1; NP_817126.1; NP_057272.1
Specificity: This antibody is expected to recognise BAF53A isoforms 1 and 2 and BAF53B, which are almost identical
Immunogen Sequence: YEEGGKQCVERKCP
Protein Name Actin-Like Protein 6b
53 Kda Brg1-Associated Factor B
Actin-Related Protein Baf53b
Arpnalpha
Brg1-Associated Factor 53b
Baf53b
Database Links Reactome: R-HSA-3214858
Reactome: R-HSA-8939243
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Actin-Like Protein 6b antibody
Anti-53 Kda Brg1-Associated Factor B antibody
Anti-Actin-Related Protein Baf53b antibody
Anti-Arpnalpha antibody
Anti-Brg1-Associated Factor 53b antibody
Anti-Baf53b antibody
Anti-ACTL6B antibody
Anti-ACTL6 antibody
Anti-BAF53B antibody

Information sourced from Uniprot.org

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