• Western blot analysis of various lysates using B4GALT1 Rabbit polyclonal antibody (STJ11104586) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-B4GALT1 antibody (50-215) (STJ11104586)

SKU:
STJ11104586

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Beta-1-4-Galactosyltransferase 1 (50-215) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: B4GALT1
Gene ID: 2683
Uniprot ID: B4GT1_HUMAN
Immunogen Region: 50-215
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 50-215 of human B4GALT1 (NP_001488.2).
Immunogen Sequence: LPQLVGVSTPLQGGSNSAAA IGQSSGELRTGGARPPPPLG ASSQPRPGGDSSPVVDSGPG PASNLTSVPVPHTTALSLPA CPEESPLLVGPMLIEFNMPV DLELVAKQNPNVKMGGRYAP RDCVSPHKVAIIIPFRNRQE HLKYWLYYLHPVLQRQQLDY GIYVIN
Tissue Specificity Ubiquitously expressed, but at very low levels in fetal and adult brain.
Post Translational Modifications The soluble form derives from the membrane forms by proteolytic processing.
Function Beta-1,4-galactosyltransferase 1: The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. Processed beta-1,4-galactosyltransferase 1: The cell surface form functions as a recognition molecule during a variety of cell to cell and cell to matrix interactions, as those occurring during development and egg fertilization, by binding to specific oligosaccharide ligands on opposing cells or in the extracellular matrix.
Protein Name Beta-1 -4-Galactosyltransferase 1
Beta-1 -4-Galtase 1
Beta4gal-T1
B4gal-T1
Beta-N-Acetylglucosaminyl-Glycolipid Beta-1 -4-Galactosyltransferase
Beta-N-Acetylglucosaminylglycopeptide Beta-1 -4-Galactosyltransferase
Lactose Synthase A Protein
N-Acetyllactosamine Synthase
Nal Synthase
Neolactotriaosylceramide Beta-1 -4-Galactosyltransferase
Udp-Gal -Beta-Glcnac Beta-1 -4-Galactosyltransferase 1
Udp-Galactose -Beta-N-Acetylglucosamine Beta-1 -4-Galactosyltransferase 1 Cleaved Into - Processed Beta-1 -4-Galactosyltransferase 1
Database Links Reactome: R-HSA-1912420
Reactome: R-HSA-2022854
Reactome: R-HSA-2534343
Reactome: R-HSA-3656244
Reactome: R-HSA-4793953
Reactome: R-HSA-5653890
Reactome: R-HSA-6798695
Reactome: R-HSA-975577
Cellular Localisation Isoform Long: Golgi Apparatus
Golgi Stack Membrane
Single-Pass Type Ii Membrane Protein
Cell Membrane
Cell Surface
Cell Projection
Filopodium
Found In Trans Cisternae Of Golgi But Is Mainly Localized At The Plasma Membrane
B4galt1 Cell Surface Expression Is Regulated By Ube2q1
Isoform Short: Golgi Apparatus
Found In Trans Cisternae Of Golgi
Processed Beta-1
4-Galactosyltransferase 1: Secreted
Soluble Form Found In Body Fluids
Alternative Antibody Names Anti-Beta-1 -4-Galactosyltransferase 1 antibody
Anti-Beta-1 -4-Galtase 1 antibody
Anti-Beta4gal-T1 antibody
Anti-B4gal-T1 antibody
Anti-Beta-N-Acetylglucosaminyl-Glycolipid Beta-1 -4-Galactosyltransferase antibody
Anti-Beta-N-Acetylglucosaminylglycopeptide Beta-1 -4-Galactosyltransferase antibody
Anti-Lactose Synthase A Protein antibody
Anti-N-Acetyllactosamine Synthase antibody
Anti-Nal Synthase antibody
Anti-Neolactotriaosylceramide Beta-1 -4-Galactosyltransferase antibody
Anti-Udp-Gal -Beta-Glcnac Beta-1 -4-Galactosyltransferase 1 antibody
Anti-Udp-Galactose -Beta-N-Acetylglucosamine Beta-1 -4-Galactosyltransferase 1 Cleaved Into - Processed Beta-1 -4-Galactosyltransferase 1 antibody
Anti-B4GALT1 antibody
Anti-GGTB2 antibody

Information sourced from Uniprot.org

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