Anti-ATP6V1B2 antibody (262-511) (STJ22729)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ATP6V1B2 (262-511) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ATP6V1B2 |
| Gene ID | 526 |
| Uniprot ID | VATB2_HUMAN |
| Immunogen Region | 262-511 |
| Immunogen Sequence | NLANDPTIERIITPRLALTT AEFLAYQCEKHVLVILTDMS SYAEALREVSAAREEVPGRR GFPGYMYTDLATIYERAGRV EGRNGSITQIPILTMPNDDI THPIPDLTGYITEGQIYVDR QLHNRQIYPPINVLPSLSRL MKSAIGEGMTRKDHADVSNQ LYACYAIGKDVQAMKAVVGE EALTSDDLLYLEFLQKFERN FIAQGPYENRTVFETLDIGW QLLRIFPKEMLKRIPQSTL |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 262-511 of human ATP6V1B2 (NP_001684.2). |
Additional Info
| Tissue Specificity | Kidney.localizes to early distal nephron, encompassing thick ascending limbs and distal convoluted tubules (at protein level). |
| Function | Non-catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. In renal intercalated cells, can partially compensate the lack of ATP6V1B1 and mediate secretion of protons (H+) into the urine under base-line conditions but not in conditions of acid load. |
| Protein Name | V-Type Proton Atpase Subunit B - Brain IsoformV-Atpase Subunit B 2Endomembrane Proton Pump 58 Kda SubunitHo57Vacuolar Proton Pump Subunit B 2 |
| Database Links | Reactome: R-HSA-1222556Reactome: R-HSA-77387Reactome: R-HSA-917977Reactome: R-HSA-9639288Reactome: R-HSA-983712Reactome: R-HSA-9857377 |
| Cellular Localisation | Apical Cell MembraneMelanosomeCytoplasmCytoplasmic VesicleSecretory VesicleSynaptic Vesicle MembranePeripheral Membrane ProteinClathrin-Coated Vesicle MembraneIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv |
| Alternative Antibody Names | Anti-V-Type Proton Atpase Subunit B - Brain Isoform antibodyAnti-V-Atpase Subunit B 2 antibodyAnti-Endomembrane Proton Pump 58 Kda Subunit antibodyAnti-Ho57 antibodyAnti-Vacuolar Proton Pump Subunit B 2 antibodyAnti-ATP6V1B2 antibodyAnti-ATP6B2 antibodyAnti-VPP3 antibody |
Information sourced from Uniprot.org