• Western blot analysis of extracts of various cell lines, using ATP6V1B2 Antibody (STJ22729) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ATP6V1B2 antibody (262-511) (STJ22729)

SKU:
STJ22729

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ATP6V1B2 (262-511) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATP6V1B2
Gene ID: 526
Uniprot ID: VATB2_HUMAN
Immunogen Region: 262-511
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 262-511 of human ATP6V1B2 (NP_001684.2).
Immunogen Sequence: NLANDPTIERIITPRLALTT AEFLAYQCEKHVLVILTDMS SYAEALREVSAAREEVPGRR GFPGYMYTDLATIYERAGRV EGRNGSITQIPILTMPNDDI THPIPDLTGYITEGQIYVDR QLHNRQIYPPINVLPSLSRL MKSAIGEGMTRKDHADVSNQ LYACYAIGKDVQAMKAVVGE EALTSDDLLYLEFLQKFERN FIAQGPYENRTVFETLDIGW QLLRIFPKEMLKRIPQSTL
Tissue Specificity Kidney.localizes to early distal nephron, encompassing thick ascending limbs and distal convoluted tubules (at protein level).
Function Non-catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. In renal intercalated cells, can partially compensate the lack of ATP6V1B1 and mediate secretion of protons (H+) into the urine under base-line conditions but not in conditions of acid load.
Protein Name V-Type Proton Atpase Subunit B - Brain Isoform
V-Atpase Subunit B 2
Endomembrane Proton Pump 58 Kda Subunit
Ho57
Vacuolar Proton Pump Subunit B 2
Database Links Reactome: R-HSA-1222556
Reactome: R-HSA-77387
Reactome: R-HSA-917977
Reactome: R-HSA-9639288
Reactome: R-HSA-983712
Cellular Localisation Apical Cell Membrane
Melanosome
Cytoplasm
Cytoplasmic Vesicle
Secretory Vesicle
Synaptic Vesicle Membrane
Peripheral Membrane Protein
Clathrin-Coated Vesicle Membrane
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Alternative Antibody Names Anti-V-Type Proton Atpase Subunit B - Brain Isoform antibody
Anti-V-Atpase Subunit B 2 antibody
Anti-Endomembrane Proton Pump 58 Kda Subunit antibody
Anti-Ho57 antibody
Anti-Vacuolar Proton Pump Subunit B 2 antibody
Anti-ATP6V1B2 antibody
Anti-ATP6B2 antibody
Anti-VPP3 antibody

Information sourced from Uniprot.org

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