Anti-ATP6V0A1 antibody (60-300) (STJ117453)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ATP6V0A1 (60-300) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ATP6V0A1 |
| Gene ID | 535 |
| Uniprot ID | VPP1_HUMAN |
| Immunogen Region | 60-300 |
| Immunogen Sequence | EMDRKLRFVEKEIRKANIPI MDTGENPEVPFPRDMIDLEA NFEKIENELKEINTNQEALK RNFLELTELKFILRKTQQFF DEMADPDLLEESSSLLEPSE MGRGTPLRLGFVAGVINRER IPTFERMLWRVCRGNVFLRQ AEIENPLEDPVTGDYVHKSV FIIFFQGDQLKNRVKKICEG FRASLYPCPETPQERKEMAS GVNTRIDDLQMVLNQTEDHR QRVLQAAAKNIRVWFIKVR |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 60-300 of human ATP6V0A1 (NP_001123493.1). |
Additional Info
| Function | Subunit of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that transports protons across cellular membranes. V-ATPase is responsible for the acidification of various organelles, such as lysosomes, endosomes, the trans-Golgi network, and secretory granules, including synaptic vesicles. In certain cell types, can be exported to the plasma membrane, where it is involved in the acidification of the extracellular environment. Required for assembly and activity of the vacuolar ATPase. Through its action on compartment acidification, plays an essential role in neuronal development in terms of integrity and connectivity of neurons. |
| Protein Name | V-Type Proton Atpase 116 Kda Subunit A 1V-Atpase 116 Kda Subunit A 1Clathrin-Coated Vesicle/Synaptic Vesicle Proton Pump 116 Kda SubunitVacuolar Adenosine Triphosphatase Subunit Ac116Vacuolar Proton Pump Subunit 1Vacuolar Proton Translocating Atpase 116 Kda Subunit A Isoform 1 |
| Database Links | Reactome: R-HSA-1222556Reactome: R-HSA-6798695Reactome: R-HSA-77387Reactome: R-HSA-917977Reactome: R-HSA-983712 |
| Cellular Localisation | Cytoplasmic VesicleClathrin-Coated Vesicle MembraneMulti-Pass Membrane ProteinSecretory VesicleSynaptic Vesicle MembraneMelanosomeIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv |
| Alternative Antibody Names | Anti-V-Type Proton Atpase 116 Kda Subunit A 1 antibodyAnti-V-Atpase 116 Kda Subunit A 1 antibodyAnti-Clathrin-Coated Vesicle/Synaptic Vesicle Proton Pump 116 Kda Subunit antibodyAnti-Vacuolar Adenosine Triphosphatase Subunit Ac116 antibodyAnti-Vacuolar Proton Pump Subunit 1 antibodyAnti-Vacuolar Proton Translocating Atpase 116 Kda Subunit A Isoform 1 antibodyAnti-ATP6V0A1 antibodyAnti-ATP6N1 antibodyAnti-ATP6N1A antibodyAnti-VPP1 antibody |
Information sourced from Uniprot.org