Anti-ATP5A1 antibody (200-300) [S5MR] (STJ11101295)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal ATP5A1 (200-300) antibody for use in WB, IHC-P, IF, ICC, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S5MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:6000IHC-P:1:200-1:2000IF/ICC:1:200-1:2000IP:0.5 Mu g-4 Mu g antibody for 400 Mu g-600 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your s |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ATP5F1A |
| Gene ID | 498 |
| Uniprot ID | ATPA_HUMAN |
| Immunogen Region | 200-300 |
| Immunogen Sequence | VPIGRGQRELIIGDRQTGKT SIAIDTIINQKRFNDGSDEK KKLYCIYVAIGQKRSTVAQL VKRLTDADAMKYTIVVSATA SDAAPLQYLAPYSGCSMGEY F |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 200-300 of human ATP5A1 (P25705). |
Additional Info
| Tissue Specificity | Fetal lung, heart, liver, gut and kidney. Expressed at higher levels in the fetal brain, retina and spinal cord. |
| Post Translational Modifications | The N-terminus is blocked. Acetylated on lysine residues. BLOC1S1 is required for acetylation. |
| Function | Subunit alpha, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain (Probable). ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro. With the catalytic subunit beta (ATP5F1B), forms the catalytic core in the F(1) domain. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites (Probable). Binds the bacterial siderophore enterobactin and can promote mitochondrial accumulation of enterobactin-derived iron ions. |
| Protein Name | Atp Synthase F(1 Complex Subunit Alpha - MitochondrialAtp Synthase F1 Subunit Alpha |
| Database Links | Reactome: R-HSA-1268020Reactome: R-HSA-163210Reactome: R-HSA-8949613Reactome: R-HSA-9837999 |
| Cellular Localisation | MitochondrionMitochondrion Inner MembranePeripheral Membrane ProteinMatrix SideCell MembraneExtracellular SideColocalizes With Hrg On The Cell Surface Of T-Cells |
| Alternative Antibody Names | Anti-Atp Synthase F(1 Complex Subunit Alpha - Mitochondrial antibodyAnti-Atp Synthase F1 Subunit Alpha antibodyAnti-ATP5F1A antibodyAnti-ATP5A antibodyAnti-ATP5A1 antibodyAnti-ATP5AL2 antibodyAnti-ATPM antibody |
Information sourced from Uniprot.org