• Western blot analysis of extracts of mouse brain, using ATP2B4 antibody (STJ112144) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-ATP2B4 antibody (1141-1205) (STJ112144)

SKU:
STJ112144

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Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ATP2B4 (1141-1205) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:4000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATP2B4
Gene ID: 493
Uniprot ID: AT2B4_HUMAN
Immunogen Region: 1141-1205
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1141-1205 of human ATP2B4 (NP_001675.3).
Immunogen Sequence: ELPRTPLLDEEEEENPDKAS KFGTRVLLLDGEVTPYANTN NNAVDCNQVQLPQSDSSLQS LETSV
Tissue Specificity Isoform XB is the most abundant isoform and is expressed ubiquitously. Isoforms containing segment Z have only been detected in heart, while isoforms containing segment a have been found in heart, stomach and brain cortex.
Function Calcium/calmodulin-regulated and magnesium-dependent enzyme that catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell. By regulating sperm cell calcium homeostasis, may play a role in sperm motility.
Protein Name Plasma Membrane Calcium-Transporting Atpase 4
Pmca4
Matrix-Remodeling-Associated Protein 1
Plasma Membrane Calcium Atpase Isoform 4
Plasma Membrane Calcium Pump Isoform 4
Database Links Reactome: R-HSA-418359
Reactome: R-HSA-5578775
Reactome: R-HSA-936837
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Cell Projection
Cilium
Flagellum Membrane
Alternative Antibody Names Anti-Plasma Membrane Calcium-Transporting Atpase 4 antibody
Anti-Pmca4 antibody
Anti-Matrix-Remodeling-Associated Protein 1 antibody
Anti-Plasma Membrane Calcium Atpase Isoform 4 antibody
Anti-Plasma Membrane Calcium Pump Isoform 4 antibody
Anti-ATP2B4 antibody
Anti-ATP2B2 antibody
Anti-MXRA1 antibody

Information sourced from Uniprot.org

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