Anti-ATP2B1 antibody (50-150) (STJ11101482)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ATP2B1 (50-150) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ATP2B1 |
| Gene ID | 490 |
| Uniprot ID | AT2B1_HUMAN |
| Immunogen Region | 50-150 |
| Immunogen Sequence | QESYGDVYGICTKLKTSPNE GLSGNPADLERREAVFGKNF IPPKKPKTFLQLVWEALQDV TLIILEIAAIVSLGLSFYQP PEGDNALCGEVSVGEEEGEG E |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human ATP2B1 (NP_001673.2). |
Additional Info
| Tissue Specificity | Isoform B: Ubiquitously expressed. Isoform C: Found in brain cortex, skeletal muscle and heart muscle. Isoform D: Has only been found in fetal skeletal muscle. Isoform K: Found in small intestine and liver. Abundantly expressed in the endometrial epithelial cells and glandular epithelial cells in early-proliferative phase and early-secretory phases. |
| Function | Catalyzes the hydrolysis of ATP coupled with the transport of calcium from the cytoplasm to the extracellular space thereby maintaining intracellular calcium homeostasis. Plays a role in blood pressure regulation through regulation of intracellular calcium concentration and nitric oxide production leading to regulation of vascular smooth muscle cells vasoconstriction. Positively regulates bone mineralization through absorption of calcium from the intestine. Plays dual roles in osteoclast differentiation and survival by regulating RANKL-induced calcium oscillations in preosteoclasts and mediating calcium extrusion in mature osteoclasts. Regulates insulin sensitivity through calcium/calmodulin signaling pathway by regulating AKT1 activation and NOS3 activation in endothelial cells. May play a role in synaptic transmission by modulating calcium and proton dynamics at the synaptic vesicles. |
| Protein Name | Plasma Membrane Calcium-Transporting Atpase 1Plasma Membrane Calcium Atpase Isoform 1Pmca1Plasma Membrane Calcium Pump Isoform 1 |
| Database Links | Reactome: R-HSA-418359Reactome: R-HSA-5578775Reactome: R-HSA-936837Reactome: R-HSA-9662360 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinBasolateral Cell MembraneSynapsePresynaptic Cell MembraneCytoplasmic VesicleSecretory VesicleSynaptic Vesicle MembraneColocalizes With Sv2a In Photoreceptor Synaptic TerminalsColocalizes With Nptn To The Immunological SynapseColocalizes With Epb41 To The Basolateral Membrane In EnterocytePreferentially Sorted To Recycling Synaptic Vesicles |
| Alternative Antibody Names | Anti-Plasma Membrane Calcium-Transporting Atpase 1 antibodyAnti-Plasma Membrane Calcium Atpase Isoform 1 antibodyAnti-Pmca1 antibodyAnti-Plasma Membrane Calcium Pump Isoform 1 antibodyAnti-ATP2B1 antibodyAnti-PMCA1 antibody |
Information sourced from Uniprot.org