• Immunohistochemistry analysis of ATP1B3 in paraffin-embedded human liver cancer using ATP1B3 Rabbit monoclonal antibody (STJ11103063) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using ATP1B3 Rabbit monoclonal antibody (STJ11103063) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ATP1B3 antibody (180-279) [S3MR] (STJ11103063)

SKU:
STJ11103063

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-ATP1B3 (180-279) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATP1B3
Gene ID: 483
Uniprot ID: AT1B3_HUMAN
Immunogen Region: 180-279
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 180-279 of human ATP1B3 (P54709).
Immunogen Sequence: GLKPEGVPRIDCVSKNEDIP NVAVYPHNGMIDLKYFPYYG KKLHVGYLQPLVAVQVSFAP NNTGKEVTVECKIDGSANLK SQDDRDKFLGRVMFKITARA
Function This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-3 subunit is not known.
Protein Name Sodium/Potassium-Transporting Atpase Subunit Beta-3
Sodium/Potassium-Dependent Atpase Subunit Beta-3
Atpb-3
Cd Antigen Cd298
Database Links Reactome: R-HSA-210991
Reactome: R-HSA-5578775
Reactome: R-HSA-936837
Reactome: R-HSA-9679191
Cellular Localisation Apical Cell Membrane
Single-Pass Type Ii Membrane Protein
Basolateral Cell Membrane
Melanosome
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Alternative Antibody Names Anti-Sodium/Potassium-Transporting Atpase Subunit Beta-3 antibody
Anti-Sodium/Potassium-Dependent Atpase Subunit Beta-3 antibody
Anti-Atpb-3 antibody
Anti-Cd Antigen Cd298 antibody
Anti-ATP1B3 antibody

Information sourced from Uniprot.org

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