• Immunohistochemistry analysis of paraffin-embedded human breast cancer using ATG7 antibody (STJ113569) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-ATG7 antibody (500-676) (STJ113569)

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STJ113569

£169.50 - £449.50
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Host: Rabbit
Applications: IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ATG7 (500-676) is suitable for use in Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATG7
Gene ID: 10533
Uniprot ID: ATG7_HUMAN
Immunogen Region: 500-676
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 500-676 of human ATG7 (NP_001129503.2).
Immunogen Sequence: LGFDTFVVMRHGLKKPKQQG AGDLCPNHPVASADLLGSSL FANIPGYKLGCYFCNDVVAP GDSTRDRTLDQQCTVSRPGL AVIAGALAVELMVSVLQHPE GGYAIASSSDDRMNEPPTSL GLVPHQVLDQYEREGFNFLA KVFNSSHSFLEDLTGLTLLH QETQAAEIWDMSDDETI
Tissue Specificity Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.
Post Translational Modifications Acetylated by EP300.
Function E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Facilitates LC3-I lipidation with phosphatidylethanolamine to form LC3-II which is found on autophagosomal membranes. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Also plays a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation. Plays a role in regulating the liver clock and glucose metabolism by mediating the autophagic degradation of CRY1 (clock repressor) in a time-dependent manner.
Protein Name Ubiquitin-Like Modifier-Activating Enzyme Atg7
Atg12-Activating Enzyme E1 Atg7
Autophagy-Related Protein 7
Apg7-Like
Hagp7
Ubiquitin-Activating Enzyme E1-Like Protein
Database Links Reactome: R-HSA-1632852
Reactome: R-HSA-6798695
Reactome: R-HSA-6802952
Reactome: R-HSA-983168
Cellular Localisation Cytoplasm
Preautophagosomal Structure
Localizes Also To Discrete Punctae Along The Ciliary Axoneme And To The Base Of The Ciliary Axoneme
Alternative Antibody Names Anti-Ubiquitin-Like Modifier-Activating Enzyme Atg7 antibody
Anti-Atg12-Activating Enzyme E1 Atg7 antibody
Anti-Autophagy-Related Protein 7 antibody
Anti-Apg7-Like antibody
Anti-Hagp7 antibody
Anti-Ubiquitin-Activating Enzyme E1-Like Protein antibody
Anti-ATG7 antibody
Anti-APG7L antibody

Information sourced from Uniprot.org

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