| Host: |
Goat |
| Applications: |
Pep-ELISA/IHC |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-ATG4A (Internal) is suitable for use in ELISA and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
IHC-Recommended concentration, 2-4µg/mlELISA-antibody detection limit dilution 1:1000. |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
ATG4A |
| Gene ID: |
115201 |
| Uniprot ID: |
ATG4A_HUMAN |
| Immunogen Region: |
Internal |
| Accession Number: |
NP_443168.2; NP_840054.1 |
| Specificity: |
This antibody is expected to recognize both reported isoforms (NP_443168.2; NP_840054.1) |
| Immunogen Sequence: |
TEENGTVNDQTFHC |
| Protein Name | Cysteine Protease Atg4aAut-Like 2 Cysteine EndopeptidaseAutophagy-Related Cysteine Endopeptidase 2Autophagin-2Autophagy-Related Protein 4 Homolog AHsapg4aHapg4a |
| Database Links | Reactome: R-HSA-1632852 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Cysteine Protease Atg4a antibodyAnti-Aut-Like 2 Cysteine Endopeptidase antibodyAnti-Autophagy-Related Cysteine Endopeptidase 2 antibodyAnti-Autophagin-2 antibodyAnti-Autophagy-Related Protein 4 Homolog A antibodyAnti-Hsapg4a antibodyAnti-Hapg4a antibodyAnti-ATG4A antibodyAnti-APG4A antibodyAnti-AUTL2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and ATG4A knockout (KO) HeLa cells, using [KO Validated] ATG4A Rabbit polyclonal antibody (STJ11100720) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89658/358156/STJ11100720_1__63349.1713122457.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and ATG4A knockout (KO) HeLa cells, using [KO Validated] ATG4A Rabbit polyclonal antibody (STJ11100720) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")