• Western blot analysis of various lysates, using ASMT rabbit polyclonal antibody (STJ28612) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ASMT antibody (1-298) (STJ28612)

SKU:
STJ28612

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ASMT (1-298) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ASMT
Gene ID: 438
Uniprot ID: ASMT_HUMAN
Immunogen Region: 1-298
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-298 of human ASMT (NP_001164510.1).
Immunogen Sequence: MGSSEDQAYRLLNDYANGFM VSQVLFAACELGVFDLLAEA PGPLDVAAVAAGVRASAHGT ELLLDICVSLKLLKVETRGG KAFYRNTELSSDYLTTVSPT SQCSMLKYMGRTSYRCWGHL ADAVREGRNQYLETFGVPAE ELFTAIYRSEGERLQFMQAL QEVWSVNGRSVLTAFDLSVF PLMCDLGGDFFKDPLPEADL YILARVLHDWADGKCSHLLE RIYHTCKPGGGILVIESLL
Tissue Specificity Expressed in the pineal gland (at protein level). In the retina, very low expression is found at the mRNA level, and not at the protein level.
Function Isoform 1: Catalyzes the transfer of a methyl group onto N-acetylserotonin, producing melatonin (N-acetyl-5-methoxytryptamine). Isoform 2: Does not show Acetylserotonin O-methyltransferase activity. Isoform 3: Does not show Acetylserotonin O-methyltransferase activity.
Protein Name Acetylserotonin O-Methyltransferase
Hydroxyindole O-Methyltransferase
Hiomt
Database Links Reactome: R-HSA-209931
Alternative Antibody Names Anti-Acetylserotonin O-Methyltransferase antibody
Anti-Hydroxyindole O-Methyltransferase antibody
Anti-Hiomt antibody
Anti-ASMT antibody

Information sourced from Uniprot.org

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