Western blot analysis of various lysates using ARHGEF3 Rabbit pAb (STJ110788) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-ARHGEF3 antibody (287-526) (STJ110788)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ110788
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General Information

Short DescriptionRabbit polyclonal ARHGEF3 (287-526) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman/Mouse
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolARHGEF3
Gene ID50650
Uniprot IDARHG3_HUMAN
Immunogen Region287-526
Immunogen SequenceLEEAINIIQGIVAEINTKTG ESECRYYKERLLYLEEGQKD SLIDSSRVLCCHGELKNNRG VKLHVFLFQEVLVITRAVTH NEQLCYQLYRQPIPVKDLLL EDLQDGEVRLGGSLRGAFSN NERIKNFFRVSFKNGSQSQT HSLQANDTFNKQQWLNCIRQ AKETVLCAAGQAGVLDSEGS FLNPTTGSRELQGETKLEQM DQSDSESDCSMDTSEVSLDC ERMEQTDSSCGNSRHGESN
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 287-526 of human ARHGEF3 (NP_062455.1).

Additional Info

Tissue Specificity Widely expressed. Highest levels are found in adult brain and skeletal muscle. Lower levels are found in heart and kidney.
Function Acts as a guanine nucleotide exchange factor (GEF) for RhoA and RhoB GTPases.
Protein Name Rho Guanine Nucleotide Exchange Factor 3
Exchange Factor Found In Platelets And Leukemic And Neuronal Tissues
Xpln
Database Links Reactome: R-HSA-193648
Reactome: R-HSA-416482
Reactome: R-HSA-8980692
Reactome: R-HSA-9013026
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Rho Guanine Nucleotide Exchange Factor 3 antibody
Anti-Exchange Factor Found In Platelets And Leukemic And Neuronal Tissues antibody
Anti-Xpln antibody
Anti-ARHGEF3 antibody

Information sourced from Uniprot.org

Citations

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