• Confocal imaging of paraffin-embedded Mouse kidney tissue using Aquaporin-1 (AQP1) Rabbit monoclonal antibody (STJ11102015, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining. Objective: 40x.
  • Confocal imaging of paraffin-embedded Mouse brain tissue using Aquaporin-1 (AQP1) Rabbit monoclonal antibody (STJ11102015, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining. Objective: 40x.
  • Confocal imaging of paraffin-embedded Human colon cancer tissue using Aquaporin-1 (AQP1) Rabbit monoclonal antibody (STJ11102015, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining. Objective: 40x.
  • Immunohistochemistry analysis of Aquaporin-1 (AQP1) in paraffin-embedded human liver using Aquaporin-1 (AQP1) Rabbit monoclonal antibody (STJ11102015) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Aquaporin-1 (AQP1) in paraffin-embedded human colon carcinoma using Aquaporin-1 (AQP1) Rabbit monoclonal antibody (STJ11102015) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Aquaporin-1 (AQP1) in paraffin-embedded human breast cancer using Aquaporin-1 (AQP1) Rabbit monoclonal antibody (STJ11102015) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Aquaporin-1 (Aquaporin-1 (AQP1) ) Rabbit monoclonal antibody (STJ11102015) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of lysates from Mouse liver, using Aquaporin-1 (Aquaporin-1 (AQP1) ) Rabbit monoclonal antibody (STJ11102015) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-AQP1 antibody (170-269) [S5MR] (STJ11102015)

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STJ11102015

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-AQP1 (170-269) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AQP1
Gene ID: 358
Uniprot ID: AQP1_HUMAN
Immunogen Region: 170-269
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 170-269 of human Aquaporin-1 (Aquaporin-1 (AQP1) ) (P29972).
Immunogen Sequence: LAIGLSVALGHLLAIDYTGC GINPARSFGSAVITHNFSNH WIFWVGPFIGGALAVLIYDF ILAPRSSDLTDRVKVWTSGQ VEEYDLDADDINSRVEMKPK
Tissue Specificity Detected in erythrocytes (at protein level). Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver.
Function Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Component of the ankyrin-1 complex, a multiprotein complex involved in the stability and shape of the erythrocyte membrane.
Protein Name Aquaporin-1
Aqp-1
Aquaporin-Chip
Urine Water Channel
Water Channel Protein For Red Blood Cells And Kidney Proximal Tubule
Database Links Reactome: R-HSA-1237044
Reactome: R-HSA-1247673
Reactome: R-HSA-432040
Reactome: R-HSA-432047
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Aquaporin-1 antibody
Anti-Aqp-1 antibody
Anti-Aquaporin-Chip antibody
Anti-Urine Water Channel antibody
Anti-Water Channel Protein For Red Blood Cells And Kidney Proximal Tubule antibody
Anti-AQP1 antibody
Anti-CHIP28 antibody

Information sourced from Uniprot.org

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