• Confocal imaging of paraffin-embedded Mouse kidney tissue using Aquaporin-1 (AQP1) Rabbit monoclonal antibody (STJ11102015, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining. Objective: 40x.
  • Confocal imaging of paraffin-embedded Mouse brain tissue using Aquaporin-1 (AQP1) Rabbit monoclonal antibody (STJ11102015, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining. Objective: 40x.
  • Confocal imaging of paraffin-embedded Human colon cancer tissue using Aquaporin-1 (AQP1) Rabbit monoclonal antibody (STJ11102015, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining. Objective: 40x.
  • Immunohistochemistry analysis of Aquaporin-1 (AQP1) in paraffin-embedded human liver using Aquaporin-1 (AQP1) Rabbit monoclonal antibody (STJ11102015) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Aquaporin-1 (AQP1) in paraffin-embedded human colon carcinoma using Aquaporin-1 (AQP1) Rabbit monoclonal antibody (STJ11102015) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Aquaporin-1 (AQP1) in paraffin-embedded human breast cancer using Aquaporin-1 (AQP1) Rabbit monoclonal antibody (STJ11102015) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Aquaporin-1 (Aquaporin-1 (AQP1) ) Rabbit monoclonal antibody (STJ11102015) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of lysates from Mouse liver, using Aquaporin-1 (Aquaporin-1 (AQP1) ) Rabbit monoclonal antibody (STJ11102015) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-AQP1 antibody (170-269) [S5MR] (STJ11102015)

SKU:
STJ11102015

$197.78 - $867.38
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:6000
IHC-P:1:500-1:2000
IF/ICC:1:100-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AQP1
Gene ID: 358
Uniprot ID: AQP1_HUMAN
Immunogen Region: 170-269
Specificity: A synthetic peptide corresponding to a sequence within amino acids 170-269 of human Aquaporin-1 (Aquaporin-1 (AQP1) ) (P29972).
Immunogen Sequence: LAIGLSVALGHLLAIDYTGC GINPARSFGSAVITHNFSNH WIFWVGPFIGGALAVLIYDF ILAPRSSDLTDRVKVWTSGQ VEEYDLDADDINSRVEMKPK
Tissue Specificity Detected in erythrocytes (at protein level). Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver.
Function Forms a water channel that facilitates the transport of water across cell membranes, playing a crucial role in water homeostasis in various tissues. Could also be permeable to small solutes including hydrogen peroxide, glycerol and gases such as amonnia (NH3), nitric oxide (NO) and carbon dioxide (CO2). Recruited to the ankyrin-1 complex, a multiprotein complex of the erythrocyte membrane, it could be part of a CO2 metabolon, linking facilitated diffusion of CO2 across the membrane, anion exchange of Cl(-)/HCO3(-) and interconversion of dissolved CO2 and carbonic acid in the cytosol. In vitro, it shows non-selective gated cation channel activity and may be permeable to cations like K(+) and Na(+) in vivo.
Protein Name Aquaporin-1
Aqp-1
Aquaporin-Chip
Channel-Like Integral Membrane Protein Of 28 Kda
Urine Water Channel
Database Links Reactome: R-HSA-1237044
Reactome: R-HSA-1247673
Reactome: R-HSA-432040
Reactome: R-HSA-432047
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Aquaporin-1 antibody
Anti-Aqp-1 antibody
Anti-Aquaporin-Chip antibody
Anti-Channel-Like Integral Membrane Protein Of 28 Kda antibody
Anti-Urine Water Channel antibody
Anti-AQP1 antibody
Anti-CHIP28 antibody

Information sourced from Uniprot.org

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