Anti-APLP1 antibody (470-649) (STJ22642)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal APLP1 (470-649) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:100-1:500IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | APLP1 |
| Gene ID | 333 |
| Uniprot ID | APLP1_HUMAN |
| Immunogen Region | 470-649 |
| Immunogen Sequence | NPHLAQELRPQIQELLHSEH LGPSELEAPAPGGSSEDKGG LQPPDSKDDTPMTLPKGSTE QDAASPEKEKMNPLEQYERK VNASVPRGFPFHSSEIQRDE LAPAGTGVSRE |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 470-580 of human APLP1 (NP_005157.1). |
Additional Info
| Tissue Specificity | Expressed in the cerebral cortex where it is localized to the postsynaptic density (PSD). |
| Post Translational Modifications | Proteolytically cleaved by caspases during neuronal apoptosis. Cleaved, in vitro, at Asp-620 by caspase-3. N- and O-glycosylated. O-glycosylation with core 1 or possibly core 8 glycans. Glycosylation on Ser-227 is the preferred site to Thr-228. |
| Function | May play a role in postsynaptic function. The C-terminal gamma-secretase processed fragment, ALID1, activates transcription activation through APBB1 (Fe65) binding. Couples to JIP signal transduction through C-terminal binding. May interact with cellular G-protein signaling pathways. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I. The gamma-CTF peptide, C30, is a potent enhancer of neuronal apoptosis. |
| Protein Name | Amyloid Beta Precursor Like Protein 1Amyloid BetaA4 Precursor-Like Protein 1Amyloid-Like Protein 1AplpAplp-1 Cleaved Into - C30 |
| Database Links | |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinC30: CytoplasmC-Terminally Processed In The Golgi Complex |
| Alternative Antibody Names | Anti-Amyloid Beta Precursor Like Protein 1 antibodyAnti-Amyloid Beta antibodyAnti-A4 Precursor-Like Protein 1 antibodyAnti-Amyloid-Like Protein 1 antibodyAnti-Aplp antibodyAnti-Aplp-1 Cleaved Into - C30 antibodyAnti-APLP1 antibody |
Information sourced from Uniprot.org