Anti-APLF antibody (200-380) (STJ11102935)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal APLF (200-380) antibody for use in WB and ELISA in rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | APLF |
| Gene ID | 200558 |
| Uniprot ID | APLF_HUMAN |
| Immunogen Region | 200-380 |
| Immunogen Sequence | LSVPAISGGNVIQGSGKEEI CKDKSQLNTTQQGRRQLISS GSSENTSAEQDTGEECKNTD QEESTISSKEMPQSFSAITL SNTEMNNIKTNAQRNKLPIE ELGKVSKHKIATKRTPHKED EAMSCSENCSSAQGDSLQDE SQGSHSESSSNPSNPETLHA KATDSVLQGSEGNKVKRTSC M |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 200-380 of human APLF (NP_775816.1). |
Additional Info
| Post Translational Modifications | Poly-ADP-ribosylated. In addition to binding non covalently poly-ADP-ribose via its PBZ-type zinc fingers, the protein is also covalently poly-ADP-ribosylated by PARP1. Phosphorylated in an ATM-dependent manner upon double-strand DNA break. |
| Function | Histone chaperone involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with branched poly-ADP-ribose chains, a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Following recruitment to DNA damage sites, acts as a histone chaperone that mediates histone eviction during DNA repair and promotes recruitment of histone variant MACROH2A1. Also has a nuclease activity: displays apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage. Together with PARP3, promotes the retention of the LIG4-XRCC4 complex on chromatin and accelerate DNA ligation during non-homologous end-joining (NHEJ). Also acts as a negative regulator of cell pluripotency by promoting histone exchange. Required for the embryo implantation during the epithelial to mesenchymal transition in females. |
| Protein Name | Aprataxin And Pnk-Like FactorApurinic-Apyrimidinic Endonuclease AplfPnk And Aptx-Like Fha Domain-Containing ProteinXrcc1-Interacting Protein 1 |
| Database Links | |
| Cellular Localisation | NucleusChromosomeCytoplasmCytosolLocalizes To Dna Damage SitesAccumulates At Single-Strand Breaks And Double-Strand Breaks Via The Pbz-Type Zinc Fingers |
| Alternative Antibody Names | Anti-Aprataxin And Pnk-Like Factor antibodyAnti-Apurinic-Apyrimidinic Endonuclease Aplf antibodyAnti-Pnk And Aptx-Like Fha Domain-Containing Protein antibodyAnti-Xrcc1-Interacting Protein 1 antibodyAnti-APLF antibodyAnti-C2orf13 antibodyAnti-PALF antibodyAnti-XIP1 antibody |
Information sourced from Uniprot.org