• Western blot analysis of lysates from Rat lung, using APLF Rabbit polyclonal antibody (STJ11102935) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-APLF antibody (200-380) (STJ11102935)

SKU:
STJ11102935

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-APLF (200-380) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: APLF
Gene ID: 200558
Uniprot ID: APLF_HUMAN
Immunogen Region: 200-380
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 200-380 of human APLF (NP_775816.1).
Immunogen Sequence: LSVPAISGGNVIQGSGKEEI CKDKSQLNTTQQGRRQLISS GSSENTSAEQDTGEECKNTD QEESTISSKEMPQSFSAITL SNTEMNNIKTNAQRNKLPIE ELGKVSKHKIATKRTPHKED EAMSCSENCSSAQGDSLQDE SQGSHSESSSNPSNPETLHA KATDSVLQGSEGNKVKRTSC M
Post Translational Modifications Poly-ADP-ribosylated. In addition to binding non covalently poly-ADP-ribose via its PBZ-type zinc fingers, the protein is also covalently poly-ADP-ribosylated by PARP1. Phosphorylated in an ATM-dependent manner upon double-strand DNA break.
Function Histone chaperone involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with branched poly-ADP-ribose chains, a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Following recruitment to DNA damage sites, acts as a histone chaperone that mediates histone eviction during DNA repair and promotes recruitment of histone variant MACROH2A1. Also has a nuclease activity: displays apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage. Together with PARP3, promotes the retention of the LIG4-XRCC4 complex on chromatin and accelerate DNA ligation during non-homologous end-joining (NHEJ). Also acts as a negative regulator of cell pluripotency by promoting histone exchange. Required for the embryo implantation during the epithelial to mesenchymal transition in females.
Protein Name Aprataxin And Pnk-Like Factor
Apurinic-Apyrimidinic Endonuclease Aplf
Pnk And Aptx-Like Fha Domain-Containing Protein
Xrcc1-Interacting Protein 1
Cellular Localisation Nucleus
Chromosome
Cytoplasm
Cytosol
Localizes To Dna Damage Sites
Accumulates At Single-Strand Breaks And Double-Strand Breaks Via The Pbz-Type Zinc Fingers
Alternative Antibody Names Anti-Aprataxin And Pnk-Like Factor antibody
Anti-Apurinic-Apyrimidinic Endonuclease Aplf antibody
Anti-Pnk And Aptx-Like Fha Domain-Containing Protein antibody
Anti-Xrcc1-Interacting Protein 1 antibody
Anti-APLF antibody
Anti-C2orf13 antibody
Anti-PALF antibody
Anti-XIP1 antibody

Information sourced from Uniprot.org

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